MORPHOMETRIC ANALYSIS OF CELLULAR INFILTRATION ASSESSED BY MONOCLONAL-ANTIBODY LABELING IN SEQUENTIAL HUMAN RENAL-ALLOGRAFT BIOPSIES
MORPHOMETRIC ANALYSIS OF CELLULAR INFILTRATION ASSESSED BY MONOCLONAL-ANTIBODY LABELING IN SEQUENTIAL HUMAN RENAL-ALLOGRAFT BIOPSIES
复制标题
DOI：
10.1097/00007890-198610000-00004
复制
发表时间：
1986-10-01
期刊：
影响因子：
6.2
通讯作者：
MORRIS, PJ
中科院分区：
文献类型：
--
作者：
MCWHINNIE, DL;THOMPSON, JF;MORRIS, PJ
使用间接免疫过氧化物酶技术通过一组单克隆抗体测定了总共 279 个人肾同种异体移植物活检中的单核细胞浸润。从 83 名随机分配接受短期环孢素 (CsA) 或常规硫唑嘌呤和低剂量泼尼松林 (AP) 治疗的患者中获得了 272 个活检组织。常规在第0天（对照活检）、第7天、第21天、第90天和第365天以及临床指示的其他时间进行活检。另外 7 名接受 AP 治疗的患者在移植几年后接受了活检（中位时间：6 年零 1 个月）。使用点计数技术对低温恒温器组织切片进行形态计量分析表明，排斥移植物中的渗透明显大于功能稳定的移植物中的渗透。然而，在功能稳定的移植物中，移植后第一周内也会出现明显的浸润。虽然这种渗透随着时间的推移而减少，但即使在移植后几年进行活检的移植物中，它仍然很重要。 CsA 治疗的浸润明显少于 AP 治疗。因此，浸润的程度随时间、移植物状态和免疫抑制而变化。相比之下，T淋巴细胞（CD3+）移植后的所有活检中，浸润的表型组成保持相对恒定，约占浸润细胞的35%，并且CD8+细胞比CD4+更常见。单核细胞和巨噬细胞占剩余浸润物的大部分。
The mononuclear cell infiltrate in a total of 279 human renal allograft biopsies was determined by a paneal of monoclonal antibodies using an indirect immunoperoxidase technique. Two hundred and seventy-two biopsies were obtained from 83 patients randomly allocated to receive short-term cyclosporine (CsA) or conventional azathioprine and low-dose prednisoline (AP). Biopsies were obtained routinely at days 0 (control biopsies), 7, 21, 90, and 365, as well as at other times when clinically indicated. A further 7 patients on AP therapy were biopsied several years after transplantation (median: 6 years 1 months). Morphometric analysis of cryostat tissue sections using a point-counting techique has shown that the infiltration in rejecting grafts is significantly greater than in grafts with stable function. However, significant infiltration also occurs within the first week after transplantation in grafts with stable function. While this infiltrate diminishes with time, it remains significant even in grafts biopsied several years after transplantation. The infiltration with CsA treatment is significantly less than with AP therapy. The magnitude of the infiltrate therefore varies with time, graft status, and immunosuppression. In contrast the phenotypic composition of the infiltrate remains relatively constant in all biopsies after transplantation with T lymphocytes (CD3+), accounting for approximately 35% of infiltrating cells and CD8+ cells more common than CD4+. Monocytes and macrophages account for most of the remainder of the infiltrate.