Detection of HTLV-1 by polymerase chain reaction in situ hybridization in adult T-cell leukemia/lymphoma.
Detection of HTLV-1 by polymerase chain reaction in situ hybridization in adult T-cell leukemia/lymphoma.
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通过聚合酶链反应原位杂交检测成人 T 细胞白血病/淋巴瘤中的 HTLV-1。
DOI：
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发表时间：
1998
影响因子：
6
通讯作者：
John J. Byrnes
中科院分区：
文献类型：
--
作者：
M. Setoyama;F. Kerdel;G. Elgart;T. Kanzaki;John J. Byrnes
应用非放射性聚合酶链反应原位杂交技术检测了成人T细胞白血病/淋巴瘤（ATLL）石蜡包埋手术标本中人T淋巴细胞病毒Ⅰ型（HTLV-Ⅰ）前病毒DNA的分布。作为对照，我们使用了5例蕈样肉芽肿的活检样本，HTLV-1感染细胞系（MT 2）的细胞，以及HTLV-1阴性细胞（YAS）。我们在90%（9/10）的ATLL病例的皮肤浸润淋巴细胞核中成功检测到HTLV-1 tax序列的扩增子。研究还发现，在ATLL患者的汗腺上皮细胞、血管内皮细胞和淋巴细胞的核中存在HTLV-1前病毒DNA。蕈样肉芽肿和YAS细胞对HTLV-I Tax序列呈阴性，但MT 2细胞呈强阳性。结果表明，这种技术是更敏感的检测细胞内扩增子比以前的原位杂交方法。通过它的使用，我们能够很容易地确定HTLV-I阳性细胞在石蜡包埋存档材料的各种细胞和组织中的分布。
A method for nonradioactive polymerase chain reaction in situ hybridization was developed and used to determine the distribution of human T-lymphotropic virus type I (HTLV-I) proviral DNA in paraffin-embedded surgical specimens of adult T-cell leukemia/lymphoma (ATLL). As controls, we used biopsy samples of five cases of mycosis fungoides, cells of an HTLV-I-infected cell line (MT2), as well as HTLV-1-negative cells (YAS). We successfully detected the amplicon of the HTLV-1 tax sequence in the nuclei of the cutaneous infiltrating lymphoid cells in 90% (9/10) of ATLL cases. Studies also revealed the existence of HTLV-1 provirus DNA in nuclei of sweat gland epithelial cells and vascular endothelial cells as well as lymphoid cells in ATLL patients. Mycosis fungoides and YAS cells were negative for the HTLV-I tax sequence, but MT2 cells were strongly positive. The results indicated that this technique was more sensitive in detecting intracellular amplicons than was the previous in situ hybridization method. Through its use, we were able to easily determine the distribution of HTLV-I-positive cells among the various cells and tissues of paraffin-embedded archival materials.
影响因子：
158.5
作者：
BAGASRA, O;HAUPTMAN, SP;POMERANTZ, RJ
通讯作者：
POMERANTZ, RJ
原位聚合酶链式反应：细胞内扩增并检测HIV-1原病毒DNA和其他特定基因。
影响因子：
2.2
作者：
Bagasra,O;Seshamma,T;Pomerantz,RJ
通讯作者：
Pomerantz,RJ
通过原位聚合酶链反应对蕈样肉芽肿患者皮肤活检中的人 T 细胞嗜淋巴细胞病毒 1 型前病毒序列进行定位。
DOI：
10.1111/1523-1747.ep12345488
发表时间：
1996
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Khan,ZM;Sebenik,M;Zucker-Franklin,D
通讯作者：
Zucker-Franklin,D