Differential T and B cell responses against Mycobacterium tuberculosis heparin-binding hemagglutinin adhesin in infected healthy individuals and patients with tuberculosis
Differential T and B cell responses against Mycobacterium tuberculosis heparin-binding hemagglutinin adhesin in infected healthy individuals and patients with tuberculosis
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DOI：
10.1086/338833
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发表时间：
2002-02-15
影响因子：
6.4
通讯作者：
Mascart, F
中科院分区：
文献类型：
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作者：
Masungi, C;Temmerman, S;Mascart, F
由于感染结核分枝杆菌的个体中只有10%最终会发病，因此被感染的健康受试者和患病受试者的免疫系统识别不同的抗原可能构成潜在的候选疫苗。在这里，肝素结合血凝素粘附素（HBHA）被确定为这样一种抗原。来自60%的健康感染者（n = 25）的淋巴细胞在HBHA刺激后产生干扰素(IFN)- γ，而活动性肺结核患者（n = 24）只有4%的淋巴细胞产生干扰素- γ。在应答者中，CD4（+）和CD8（+）细胞均分泌hbha特异性ifn - γ，抗原由主要组织相容性复合体I类和II类分子呈递。与结核患者产生hbha特异性ifn - γ的能力降低相反，他们中的大多数（82%）产生抗hbha抗体，而感染健康受试者的这一比例为36%。这些观察结果表明，HBHA在结核病患者和受感染的健康个体的免疫系统中的识别方式不同，可能为预防结核病提供了一种标记。
Because only 10% of individuals infected with Mycobacterium tuberculosis will eventually develop disease, antigens that are recognized differently by the immune systems of infected healthy and diseased subjects may constitute potential vaccine candidates. Here, the heparin-binding hemagglutinin adhesin (HBHA) is identified as such an antigen. Lymphocytes from 60% of healthy infected individuals (n = 25) produced interferon (IFN)-gamma after stimulation with HBHA, compared with only 4% of patients with active tuberculosis (n = 24). In the responders, both CD4(+) and CD8(+) cells secreted HBHA-specific IFN-gamma, and the antigen was presented by both major histocompatibility complex class I and II molecules. In contrast to the reduced ability of patients with tuberculosis to produce HBHA-specific IFN-gamma, most of them (82%) produced anti-HBHA antibodies, compared with 36% of the infected healthy subjects. These observations indicate that HBHA is recognized differently by the immune systems of patients with tuberculosis and infected healthy individuals and might provide a marker for protection against tuberculosis.