Biosynthetic Gene Expression and Tissue Distribution of Diosgenin in Dioscorea japonica
Biosynthetic Gene Expression and Tissue Distribution of Diosgenin in Dioscorea japonica
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薯蓣皂苷元生物合成基因表达及组织分布
DOI：
10.1021/acs.jafc.2c08478
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发表时间：
2023
影响因子：
6.1
通讯作者：
Miyoshi Noriyuki
中科院分区：
文献类型：
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作者：
Onoda Keita;Kato Mai;Tsunematsu Yuta;Eto Fumihiro;Sato Michio;Yoshioka Yasukiyo;Yoshida Takuya;Tamura Kentaro;Yao Ikuko;Dohra Hideo;Watanabe Kenji;Miyoshi Noriyuki
薯蓣皂苷元是薯蓣皂苷的苷元，薯蓣皂苷是在植物中发现的一种主要生物活性甾体皂苷，包括喜马拉雅重楼 (Paris polyphylla)、胡芦巴 (Trigonella foenum-graecum) 和山药 (Dioscoreaspp.)。我们之前已经证明，一种天然山药，薯蓣，含有一种有前途的生物活性化合物薯蓣皂苷元，它具有抗癌和抗高三酰甘油活性。在这里，我们首次发现日本山药（D. japonica）珠芽中的薯蓣皂苷元含量比可食用块茎（根茎）和叶子更丰富。 LC-MS 和成像 MS 分析表明薯蓣皂苷元在 D 的周围区域积累。粳稻球茎。此外，我们对D进行了RNA-seq分析。粳稻，并鉴定了多序列比对D. japonicaCYP90(DjCYP90)，是 CYP90G4 inP 的直系同源基因。重楼，CYP90B50 inT。 Foenum-graecum、薯蓣中的 CYP90G6 和长毛薯蓣中的 CYP90G，编码薯蓣皂苷元生物合成限速酶。 DjCYP90 的表达水平显着上调。粳稻球茎多于其根茎和叶。由于薯蓣皂苷元是最有前途的功能性食品因子之一，具有多种有利的生物活性，D.富含薯蓣皂苷元的粳豆将是一种有益的自然资源。
Diosgenin is an aglycone of dioscin, a major bioactive steroidal saponin found in plants, including Himalayan Paris (Paris polyphylla), fenugreek (Trigonella foenum-graecum), and yam (Dioscoreaspp.). We have previously demonstrated that a species of natural yam,Dioscorea japonica, contains a promising bioactive compound diosgenin, which induces anti-carcinogenic and anti-hypertriacylglycerolemic activities. Here, we found for the first time that Japanese yam (D. japonica) bulbils are richer in diosgenin than the edible tubers (rhizomes) and leaves. LC–MS and imaging-MS analyses revealed that diosgenin accumulated in the peripheral region ofD. japonicabulbils. Additionally, we performed RNA-seq analysis ofD. japonica, and multiple sequence alignment identifiedD. japonicaCYP90(DjCYP90), the orthologous gene of CYP90G4 inP. polyphylla, CYP90B50 inT. foenum-graecum, CYP90G6 inDioscorea zingiberensis, and CYP90G inDioscorea villosa, which encodes a diosgenin biosynthetic rate-limiting enzyme. The expression levels ofDjCYP90 were significantly upregulated inD. japonicabulbils than in its rhizomes and leaves. Since diosgenin is one of the most promising functional food factors executing several favorable bioactivities,D. japonicabulbils rich in diosgenin would be a beneficial natural resource.