TOLERANCE OF SOIL ACIDITY IN SYMBIOSES OF MUNG BEAN WITH RHIZOBIA
TOLERANCE OF SOIL ACIDITY IN SYMBIOSES OF MUNG BEAN WITH RHIZOBIA
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DOI：
10.2134/agronj1979.00021962007100020010x
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发表时间：
1979-01-01
期刊：
影响因子：
2.1
通讯作者：
WHITACRE, KW
中科院分区：
文献类型：
--
作者：
MUNNS, DN;KEYSER, HH;WHITACRE, KW
在温室试验中，40株根瘤菌作为单独的种子接种剂(5倍)测定了它们对土壤酸性的耐受性。104个细胞/粒)对绿豆(Vigna Radiata L.)并通过寄主植物的结瘤、生长和氮素产量来衡量其表现。这些植物生长在低氮、低钙酸性底土、戈尔德里奇细砂壤土(典型的单壤土、细壤土、混合土、中壤土)上，保持其自然pH值为5.0(饱和糊状)或用CaCO3石灰浸泡至pH 6.3。每个pH.次。菌株处理在单独的盆栽中进行三倍。菌株在耐酸性方面表现出很大的，甚至可能是连续的变异。有几个非常敏感：它们在pH值为5.0的条件下不能结瘤。大约一半是中度敏感的：在pH值为5.0时，结瘤和生长受到严重损害。其余的是耐性的：与NH4NO3一样，它们在两种土壤pH值下都支持相似的植物生长。有些菌株将高耐受性和高有效性结合在一起。S菌株的耐酸性不能从它在适宜的pH下结节的丰度或有效性，也不能根据它在常规酵母甘露醇培养基中的生长速度或产酸能力来预测。少数菌株对一个寄主品种敏感，而对另一个寄主品种耐酸，这意味着在只使用一种接种剂的试验中，不能有效地比较共生豆科植物的耐酸性。
Variation in tolerance of soil acidity among 40 rhizobial strains was assessed in greenhouse trials in which the strains were applied as separate seed inoculants (5 .times. 104 cells/seed) to 2 cultivars of mung bean (Vigna radiata L.) and performance was measured by nodulation, growth and N yield of the host plant. The plants grew in a low N, low Ca acid subsoil, Goldridge fine sandy loam (Typic Hapludult, fine loamy, mixed, mesic), left at its natural pH of 5.0 (saturation paste) or limed with CaCO3 to pH 6.3. Each pH .times. strain treatment was triplicated in separate pots. Strains demonstrated a large and perhaps continuous variation in acid tolerance. A few were very sensitive: they failed to nodulate at pH 5.0. About half were moderately sensitive: nodulation and growth were significantly impaired at pH 5.0. The remainder were tolerant: like NH4NO3 they supported similar plant growth at both soil pH values. Some strains combined high tolerance with high effectiveness. A strain''s acid tolerance could not be predicted from the abundance or effectiveness with which it nodulated at favorable pH, or from its growth rate or acid production in conventional yeast mannitol medium. A few strains were sensitive on one host cultivar and tolerant on the other, implying that acid tolerances of symbiotic legumes cannot be compared validly in trials with only 1 inoculant.