THE EXPRESSION OF THE WILMS-TUMOR GENE, WT1, IN THE DEVELOPING MAMMALIAN EMBRYO
THE EXPRESSION OF THE WILMS-TUMOR GENE, WT1, IN THE DEVELOPING MAMMALIAN EMBRYO
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DOI：
10.1016/0925-4773(93)90090-k
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发表时间：
1993-01-01
影响因子：
2.6
通讯作者：
BARD, JBL
中科院分区：
文献类型：
--
作者：
ARMSTRONG, JF;PRITCHARDJONES, K;BARD, JBL
在发育中的小鼠中，Wilms的肿瘤基因WT1首先在体腔外侧的中间间充质中表达(13个体节，早期9个DPC胚胎)。几个小时后，它出现在所有的腔周围和泌尿生殖道脊(最早的中肾小管)和分化的心脏间皮细胞中。到11DPC时，在未诱导的后肾间充质和脊髓的运动神经元中都有表达。在12.5DPC时，WT1在诱导的肾间充质中的表达增加，一天后在肾源性凝聚中尤其明显。在13.5DPC时，WT1在一些分化的体壁肌肉组织中短暂表达，而两天后，在第四脑室顶端有一个小的表达区域。然而，到第20天时，表达已局限于肾小球。对12.5个DPC胚胎和成体组织的RNA-PCR分析表明，WT1在眼和舌中都有微弱的表达。人胚胎(28-70天)的表达模式与相应的小鼠胚胎(10-15天)的表达模式非常相似。结果表明，WT1主要存在于中胚层来源的组织中，但外胚层来源的脊髓和脑组织除外。这些数据表明，WT1在一些间质向上皮间充质转化的过程中发挥了作用，但它在其他地方的表达表明，它在发育中还有其他组织特异性的作用。
In the developing mouse, the Wilms' tumour gene, WT1, is first expressed in the intermediate mesenchyme lateral to the coelomic cavity (13 somite, early 9 dpc embryo). A few hours later, it is present around all the cavity and in the urogenital ridge (the earliest mesonephric tubules) and the differentiating heart mesothelium. By 11 dpc, expression is in the uninduced metanephric mesenchyme and in the presumptive motor neurons of the spinal cord. By 12.5 dpc, WT1 expression has increased in the induced mesenchyme of the kidney and a day later is particularly marked in the nephrogenic condensations. At 13.5 dpc, WT1 is briefly expressed in some differentiating body-wall musculature, while two days later, there is a small domain of expression in the roof of the fourth ventricle of the brain. By day 20, however, expression has become restricted to the kidney glomeruli. RNA-PCR analysis on 12.5 dpc embryos and on adult tissues shows that WT1 is weakly expressed in both eye and tongue. The expression pattern in human embryos (28-70 days) is very similar to that in the equivalent mouse stages (10-15 dpc). The results indicate that WT1 is mainly present in mesodermally derived tissues, although exceptions are ectodermally derived spinal cord and brain. The data indicate that WT1 plays a role in mediating some cases of the mesenchyme-to-epithelial transition, but its expression elsewhere argues that it has other tissue-specific roles in development.