Genome Editing of Rat
Genome Editing of Rat
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大鼠基因组编辑
DOI：
10.1007/978-1-0716-3016-7_17
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发表时间：
2023
影响因子：
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通讯作者：
Takehitio Kaneko
中科院分区：
文献类型：
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作者：
依馬朋香;村田知弥;岡村永一;三上夏輝;松本翔馬;水野聖哉;依馬正次;杉山文博;Takehitio Kaneko
随着基因组编辑技术的发展，已经产生了许多基因工程大鼠品系，但过去技术难度大、生产效率低。使用锌指核酸酶 (ZFN)、类转录激活子效应核酸酶 (TALEN) 或成簇规则间隔短回文重复序列 (CRISPR)-Cas9 可以简单快速地产生敲除和敲入菌株。目前，基因组编辑菌株已通过显微注射和一种新的电穿孔方法产生，称为电穿孔动物敲除系统技术（TAKE）。本章介绍了生产基因组编辑大鼠的最新方案。
Many genetically engineered rat strains have been produced by the development of genome editing technology, although it used to be technical difficulty and low production efficiency. Knockout and knock-in strains can be simple and quick produced using zinc finger nuclease (ZFN),transcription activator-like effector nuclease(TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Presently, genome edited strains have been produced by microinjection and a new electroporation method named technique for animal knockout system byelectroporation(TAKE). This chapter presents the latest protocols for producing genome edited rats.