Isolation and molecular phylogenetic analysis of actin-coding regions from Emiliania huxleyi, a Prymnesiophyte alga, by reverse transcriptase and PCR methods.
Isolation and molecular phylogenetic analysis of actin-coding regions from Emiliania huxleyi, a Prymnesiophyte alga, by reverse transcriptase and PCR methods.
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通过逆转录酶和 PCR 方法对来自 Emiliania huxleyi（一种 Prymnesiophyte 藻类）的肌动蛋白编码区进行分离和分子系统发育分析。
DOI：
10.1093/oxfordjournals.molbev.a040021
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发表时间：
1993
影响因子：
10.7
通讯作者：
Sogin,ML
中科院分区：
文献类型：
--
作者：
Bhattacharya,D;Stickel,SK;Sogin,ML
用逆转录和聚合酶链反应方法从含叶绿素a + C的单细胞植物Emilianiahuxleyi（Prymnesiophyta）中扩增和克隆肌动蛋白cDNA。E. Huxleyi由含有至少六个不同编码区的基因家族定义，所述编码区来源于相对较新的基因重复。5个编码区（1、2和4-6型）仅在同义密码子之间变化。第六个编码区（3型肌动蛋白）的一个非同义变化产生了丝氨酸到苯丙氨酸的替换。E.第三位的G + C成分。huxleyi actin基因的G + C含量为98%，这与第一和第二位置的50% G + C含量的平均值形成对比。距离矩阵和简约性分析的肌动蛋白基因确定prymnesiophytes作为一个光合谱系，是不是已经与其他真核藻类群体。
Reverse transcriptase and polymerase chain reaction methods were used to amplify and clone actin cDNAs from the chlorophylls a + C-containing unicellular alga, Emiliania huxleyi (Prymnesiophyta). Actins in E. huxleyi are defined by a gene family containing at least six distinct coding regions that were derived from relatively recent gene duplications. Five of the coding regions (types 1, 2, and 4-6) varied only among synonymous codons. A nonsynonomous change in a sixth coding region (type 3 actin) produced a serine-to-phenylalanine replacement. The G + C composition of third positions in E. huxleyi actin genes is 98%, which contrasts with the mean value of 50% G + C content for first and second positions. Distance-matrix and parsimony analyses of actin genes identified the prymnesiophytes as a photosynthetic lineage that is not already related to other eukaryotic algal groups.