Reversible unfolding of single RNA molecules by mechanical force
Reversible unfolding of single RNA molecules by mechanical force
复制标题
DOI：
10.1126/science.1058498
复制
发表时间：
2001-04-27
期刊：
影响因子：
56.9
通讯作者：
Bustamante, C
中科院分区：
文献类型：
--
作者：
Liphardt, J;Onoa, B;Bustamante, C
在这里，我们使用机械力诱导单个 RNA 分子的展开和重折叠：一个简单的 RNA 发夹、一个包含三螺旋连接的分子以及嗜热四膜虫核酶的 P5abc 结构域。所有三个分子（P5abc 仅在不存在 Mg2+ 的情况下）都可以在平衡状态下机械展开，并且当在临界力范围内保持恒定力时。是双稳态的，并且在折叠和展开状态之间跳跃。我们确定了折叠/展开这些单个 RNA 分子的力依赖性平衡常数以及它们沿反应坐标的过渡态位置。
Here we use mechanical force to induce the unfolding and refolding of single RNA molecules: a simple RNA hairpin, a molecule containing a three-helix junction, and the P5abc domain of the Tetrahymena thermophila ribozyme. All three molecules (P5abc only in the absence of Mg2+) can be mechanically unfolded at equilibrium, and when kept at constant force within a critical force range. are bi-stable and hop between folded and-unfolded states. We determine the force-dependent equilibrium constants for folding/unfolding these single RNA molecules and the positions of their transition states along the reaction coordinate.