Neural and mammary gland defects in ErbB4 knockout mice genetically rescued from embryonic lethality
Neural and mammary gland defects in ErbB4 knockout mice genetically rescued from embryonic lethality
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DOI：
10.1073/pnas.1436402100
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发表时间：
2003-07-08
影响因子：
11.1
通讯作者：
Golding, JP
中科院分区：
文献类型：
--
作者：
Tidcombe, H;Jackson-Fisher, A;Golding, JP
缺乏表皮生长因子受体家族成员 ErbB4 的小鼠表现出颅神经嵴细胞迁移缺陷，但由于心脏发育缺陷，在胚胎第 11 天就死亡。为了检查后来的表型，我们通过在心脏特异性肌球蛋白启动子下表达 ErbB4 来挽救 ErbB4 突变小鼠的心脏缺陷。获救的 Erb84 突变小鼠已成年并具有生育能力。然而，在怀孕期间，乳腺小叶泡无法正确分化，泌乳功能也有缺陷。获救的小鼠还表现出异常的脑神经结构和小脑内大型中间神经元数量的增加。
Mice lacking the epidermal growth factor receptor family member ErbB4 exhibit defects in cranial neural crest cell migration but die by embryonic day 11 because of defective heart development. To examine later phenotypes, we rescued the heart defects in ErbB4 mutant mice by expressing ErbB4 under a cardiac-specific myosin promoter. Rescued Erb84 mutant mice reach adulthood and are fertile. However, during pregnancy, mammary lobuloalveoli fail to differentiate correctly and lactation is defective. Rescued mice also display aberrant cranial nerve architecture and increased numbers of large interneurons within the cerebellum.