Generation and identification of tumor-evoked regulatory B cells.
Generation and identification of tumor-evoked regulatory B cells.
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DOI：
10.1007/978-1-4939-1161-5_19
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发表时间：
2014
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尽管有充分的证据表明Bregs对自我反应和保护免受有害自身免疫的影响，但对其在癌症中的作用仍知之甚少。我们最近发现了一种独特的由乳腺癌引起的调节B细胞亚群，以介导转移癌细胞的保护。这些结果与过去40年关于B细胞在肿瘤发生中的丰富发现一起表明，存在额外的癌症Bregs调节抗癌反应。为了促进对它们的研究，我们在这里提供了肿瘤诱发调节性B细胞的表征和生成的详细方案。在适用的情况下，我们还讨论Breg研究在癌症中的细微差别和独特性，以警告潜在的陷阱。
The involvement of Bregs in cancer remains poorly understood despite their well-documented regulation of responses to the self and protection from harmful autoimmunity. We recently discovered a unique regulatory B cell subset evoked by breast cancer to mediate protection of metastasizing cancer cells. These results together with the wealth of findings of the last 40 years on B cells in tumorigenesis suggest the existence of additional cancer Bregs modulating anticancer responses. To facilitate the search for them, here we provide our detailed protocol for the characterization and generation of tumor-evoked regulatory B cells. Wherever applicable, we also discuss nuances and uniqueness of a Breg study in cancer to warn potential pitfalls.