Synergistic Chemo-Photothermal Therapy of Breast Cancer by Mesenchymal Stem Cell-Encapsulated Yolk-Shell GNR@HPMO-PTX Nanospheres.
Synergistic Chemo-Photothermal Therapy of Breast Cancer by Mesenchymal Stem Cell-Encapsulated Yolk-Shell GNR@HPMO-PTX Nanospheres.
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间充质干细胞包裹卵黄壳 GNR@HPMO-PTX 纳米球协同化疗光热治疗乳腺癌
DOI：
10.1021/acsami.6b05677
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Lu Guangming
中科院分区：
文献类型：
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作者：
Wu Jiang;Liu Ying;Tang Yuxia;Wang Shouju;Wang Chunyan;Li Yanjun;Su Xiaodan;Tian Jihong;Tian Ying;Pan Jing;Su Yunyan;Zhu Hong;Teng Zhaogang;Lu Guangming
间充质干细胞(MSCs)作为肿瘤治疗的载体越来越受到人们的关注。在此，首次提出了基于MSC的协同肿瘤治疗策略。为了实现这一目标，首先制备了蛋黄壳层结构的金纳米棒嵌入中空周期介孔有机二氧化硅纳米球(GNR@HPMOs)，该纳米球具有较高的紫杉醇(PTX)负载能力和良好的近红外光照射下的光热传递能力。细胞毒性和迁移实验表明，高含量的PTX负载的GNR@HPMOS(简称GNR@HPMOS-PTX)内化后，MSCs的活性和肿瘤归巢能力被很好地保留下来。体外实验表明，GNR@HPMOS-PTX负载的MSCs(GNR@HPMOS-PTX@MSCs)对乳腺癌细胞具有协同的化学-光热杀伤作用。此外，光声成像显示，肿瘤内注射后，MSCs可以改善GNR@HPMOS-PTX在肿瘤组织中的分散和分布。裸鼠乳腺癌模型体内实验进一步证明，GNR@HPMOS-PTX@MSCs对肿瘤生长有明显的抑制作用，提示其在肿瘤协同治疗方面具有巨大潜力。
Mesenchymal stem cells (MSCs) have attracted increasing attention as vehicles for cancer treatment. Herein, MSC-based synergistic oncotherapy strategy is presented for the first time. To achieve this goal, yolk-shell structured gold nanorod embedded hollow periodic mesoporous organosilica nanospheres (GNR@HPMOs) with high paclitaxel (PTX) loading capability and excellent photothermal transfer ability upon near-infrared (NIR) light irradiation are first prepared. Cytotoxicity and migration assays show that the viability and tumor-homing capability of MSCs are well-retained after internalization of high content of PTX loaded GNR@HPMOs (denoted as GNR@HPMOs-PTX). In vitro experiments show the GNR@HPMOs-PTX loaded MSCs (GNR@HPMOs-PTX@MSCs) possess synergistic chemo-photothermal killing effects for breast cancer cells. Also, photoacoustic imaging shows that the MSCs can improve dispersion and distribution in tumor tissue for GNR@HPMOs-PTX after intratumoral injection. In vivo experiments in breast cancer model of nude mice further demonstrate that the GNR@HPMOs-PTX@MSCs significantly inhibit tumor growth, suggesting their great potential for synergistic therapy of cancer.