Protective effect of taurine on the light‐induced disruption of isolated frog rod outer segments
Protective effect of taurine on the light‐induced disruption of isolated frog rod outer segments
复制标题
DOI：
10.1002/jnr.490060309
复制
发表时间：
1981
影响因子：
4.2
通讯作者：
H. Pasantes‐Morales;R. M. Ademe;Quesada;Centro de lnvestigaciones en;Fisiologfa Celular;A. Postal
中科院分区：
文献类型：
--
作者：
H. Pasantes‐Morales;R. M. Ademe;Quesada;Centro de lnvestigaciones en;Fisiologfa Celular;A. Postal
在Krebs-碳酸氢盐培养基中孵育并照射2小时的分离的蛙视杆外节（ROS）显示其结构发生了深刻的变化。其特征是椎间盘膨胀、水疱和明显肿胀。光诱导的ROS破坏需要碳酸氢盐和氯化钠的存在。用TRIS或HEPES替代碳酸氢盐保护ROS结构。此外，用蔗糖或氯化胆碱取代氯化钠保持ROS结构不变。钙、镁或磷酸盐的缺失不会改变照明产生的效果。与黑暗中的对照相比，在照射的ROS中观察到标记的碳酸氢盐和氚化水的积累增加。浓度为5-25 mM的牛磺酸、GABA或甘氨酸的存在可有效抵消光诱导的ROS破坏。牛磺酸（25 mM）将标记的碳酸氢盐和氚化水水平降低至在黑暗孵育的ROS中观察到的水平。
Isolated frog rod outer segments (ROS) incubated in a Krebs‐bicarbonate medium, and illuminated for 2 h, show a profound alteration in their structure. This is characterized by distention of discs, vesiculation, and a marked swelling. The light‐induced ROS disruption requires the presence of bicarbonate and sodium chloride. Replacement of bicarbonate by TRIS or HEPES protects ROS structure. Also, substitution of sodium chloride by sucrose or choline chloride maintains unaltered the ROS structure. Deletion of calcium, magnesium, or phosphate does not modify the effect produced by illumination. An increased accumulation of labeled bicarbonate and tritiated water is observed in illuminated ROS, as compared with controls in the dark. The presence of taurine, GABA, or glycine, at concentrations of 5–25 mM, effectively counteracts the light‐induced ROS disruption. Taurine (25 mM) reduces labeled bicarbonate and tritiated water levels to those observed in the dark incubated ROS.