Glycosylation of Secondary Metabolites: A Multifunctional UDP-Glycosyltransferase, CsUGT74Y1, Promotes the Growth of Plants.
Glycosylation of Secondary Metabolites: A Multifunctional UDP-Glycosyltransferase, CsUGT74Y1, Promotes the Growth of Plants.
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DOI：
10.1021/acs.jafc.3c05843
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发表时间：
2023-11
影响因子：
6.1
通讯作者：
Changli Yang;Fengyun Tian;Jie Ma;Mei Chen;Xingxing Shi;Dingli Chen;Youshudi Xie;Xingrong Zhou-Xing
中科院分区：
文献类型：
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作者：
Changli Yang;Fengyun Tian;Jie Ma;Mei Chen;Xingxing Shi;Dingli Chen;Youshudi Xie;Xingrong Zhou-Xing
茶树含有许多糖基化的次级代谢产物，为植物和人类提供各种益处。然而，催化茶树多种代谢产物糖基化的基因尚不清楚。在这里，180尿苷二磷酸依赖性糖基转移酶，可能参与糖基化的次生代谢产物的生物合成，从国家生物技术信息中心的公共数据库中确定。随后，通过系统发育分析和基因表达谱分析筛选CsUGT 74 Y1。组成和诱导表达分析表明，CsUGT 74 Y1在茶树嫩梢中高表达，并在生物和非生物胁迫条件下诱导表达。体外酶促分析表明，rCsUGT 74 Y1编码一个多功能的UGT，催化类黄酮，酚酸，木质素和生长素的糖基化。此外，CsUGT 74 Y1过表达拟南芥表现出增强的生长和积累的黄酮醇和生长素葡萄糖苷。我们的研究结果为识别特定的UGT提供了见解，并证明CsUGT 74 Y1是一种多功能的UGT，可促进植物发育。
Camellia sinensis contains numerous glycosylated secondary metabolites that provide various benefits to plants and humans. However, the genes that catalyze the glycosylation of multitype metabolites in tea plants remain unclear. Here, 180 uridine diphosphate-dependent glycosyltransferases that may be involved in the biosynthesis of glycosylated secondary metabolites were identified from the National Center for Biotechnology Information public databases. Subsequently, CsUGT74Y1 was screened through phylogenetic analysis and gene expression profiling. Compositional and induced expression analyses revealed that CsUGT74Y1 was highly expressed in tea tender shoots and was induced under biotic and abiotic stress conditions. In vitro enzymatic assays revealed that rCsUGT74Y1 encoded a multifunctional UGT that catalyzed the glycosylation of flavonoids, phenolic acids, lignins, and auxins. Furthermore, CsUGT74Y1-overexpressing Arabidopsis thaliana exhibited enhanced growth and accumulation of flavonol and auxin glucosides. Our findings provide insights into identifying specific UGTs and demonstrate that CsUGT74Y1 is a multifunctional UGT that promotes plant development.