Activation and fusion induced by 1 alpha,25-dihydroxyvitamin D3 and their relation in alveolar macrophages.
Activation and fusion induced by 1 alpha,25-dihydroxyvitamin D3 and their relation in alveolar macrophages.
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1α,25-二羟基维生素 D3 诱导的激活和融合及其在肺泡巨噬细胞中的关系。
DOI：
10.1073/pnas.81.22.7112
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发表时间：
1984
影响因子：
11.1
通讯作者：
Suda,T
中科院分区：
文献类型：
--
作者：
Abe,E;Shiina,Y;Miyaura,C;Tanaka,H;Hayashi,T;Kanegasaki,S;Saito,M;Nishii,Y;DeLuca,HF;Suda,T
1 α,25-二羟基维生素 D3 [1 α,25-(OH)2D3] 诱导小鼠肺泡巨噬细胞融合。在含有 5% 人血清的生长培养基中观察到这种效应，但在含有 5% 胎牛血清的培养基中观察不到这种效应。与 1 α,25-(OH)2D3 不同，细菌脂多糖 (LPS) 不会诱导肺泡巨噬细胞的融合。然而，通过葡萄糖消耗、Fc 受体增加和细胞毒性诱导来测量，1 α,25-(OH)2D3 和 LPS 均激活肺泡巨噬细胞。 1α,25-(OH)2D3诱导的多核巨细胞表面Fc受体数量远小于激素处理的单核巨噬细胞表面Fc受体数量。这些结果表明1α,25-(OH)2D3诱导肺泡巨噬细胞的融合和激活，而LPS仅引起激活。
1 alpha,25-Dihydroxyvitamin D3 [1 alpha,25-(OH)2D3] induces fusion of murine alveolar macrophages. This effect was observed in growth medium containing 5% human serum but not in the medium with 5% fetal bovine serum. Unlike 1 alpha,25-(OH)2D3, bacterial lipopolysaccharides (LPS) did not induce fusion of alveolar macrophages. However, both 1 alpha,25-(OH)2D3 and LPS activated alveolar macrophages, as measured by glucose consumption, increase in Fc receptors, and induction of cytotoxicity. The number of Fc receptors on the surface of multinucleated giant cells induced by 1 alpha,25-(OH)2D3 was much smaller than that on the surface of mononuclear macrophages treated with the hormone. These results indicate that 1 alpha,25-(OH)2D3 induces both fusion and activation of alveolar macrophages, whereas LPS elicits activation only.