Expression of zebrafish glutamate receptor δ2 in neurons with cerebellum-like wiring
Expression of zebrafish glutamate receptor δ2 in neurons with cerebellum-like wiring
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DOI：
10.1016/j.bbrc.2004.07.095
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发表时间：
2004-09-10
影响因子：
3.1
通讯作者：
Mishina, M
中科院分区：
文献类型：
--
作者：
Mikami, Y;Yoshida, T;Mishina, M
哺乳动物谷氨酸受体（GluR）δ 2选择性表达于小脑浦肯野细胞，在小脑可塑性、运动学习和神经布线中起关键作用。在这里，我们分离的cDNA编码的斑马鱼直系同源的哺乳动物GluRdelta2。我们发现，在成年斑马鱼脑中，glurdelta2 mRNA不仅表达在小脑浦肯野细胞，但也在嵴细胞的内侧octavolateral核（MON）和I型神经元的视顶盖。免疫组织化学分析显示，斑马鱼GluRdelta2蛋白选择性地定位于这些神经元的顶端树突。有趣的是，MON的嵴细胞和视顶盖的I型神经元在顶树突处接收大量平行纤维输入，在近端或基底树突处接收感觉输入。这些结果表明，斑马鱼GluRdelta2的表达是选择性的小脑样神经布线与大量的平行纤维输入。(C)2004年爱思唯尔公司All rights reserved.
Mammalian glutamate receptor (GluR) delta2 is selectively expressed in cerebellar Purkinje cells and plays key roles in cerebellar plasticity, motor learning, and neural wiring. Here, we isolated cDNA encoding the zebrafish ortholog of mammalian GluRdelta2. We found that in adult zebrafish brain, glurdelta2 mRNA was expressed not only in cerebellar Purkinje cells, but also in the crest cells of the medial octavolateral nucleus (MON) and the type I neurons of the optic tectum. Immunohistochemical analysis revealed that zebrafish GluRdelta2 proteins were selectively localized in the apical dendrites of these neurons. Interestingly, the crest cells of the MON and the type I neurons of the optic tectum receive large numbers of parallel fiber inputs at the apical dendrites and sensory inputs at the proximal or basal dendrites. These results suggest that the expression of zebrafish GluRdelta2 is selective for cerebellum-like neural wiring with large numbers of parallel fiber inputs. (C) 2004 Elsevier Inc. All rights reserved.