PROMOTERS OF ESCHERICHIA-COLI - A HIERARCHY OF INVIVO STRENGTH INDICATES ALTERNATE STRUCTURES
PROMOTERS OF ESCHERICHIA-COLI - A HIERARCHY OF INVIVO STRENGTH INDICATES ALTERNATE STRUCTURES
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DOI：
10.1002/j.1460-2075.1986.tb04596.x
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发表时间：
1986-11-01
期刊：
影响因子：
11.4
通讯作者：
BUJARD, H
中科院分区：
文献类型：
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作者：
DEUSCHLE, U;KAMMERER, W;BUJARD, H
14个启动子的体内强度是在一个系统中测定的，该系统允许高精度地定量RNA合成。在启动子强度上的差异高达75倍，最有效的信号是来自T7和T5噬菌体的启动子。它们的活性接近完全诱导的rRNA操纵子启动子的强度，这可能接近单个序列的功能最佳。相比之下，人工合成的“共识推动者”属于效率较低的信号。我们的数据表明，最佳的启动子功能可以通过交替的结构实现，并强烈表明“经典”启动子区域之外的信息有助于启动子的活性。
The strength in vivo of 14 promoters was determined in a system which permits the quantitation of RNA synthesis with high accuracy. Up to 75-fold differences in promoter strength were measured and the most efficient signals are promoters from coliphages T7 and T5. Their activity approaches the strength of fully induced promoters of the rRNA operons which may be close to the functional optimum of a single sequence. By contrast, a synthetic ''consensus promoter'' belongs to the less efficient signals. Our data show that optimal promoter function can be achieved by alternate structures and strongly suggest that information outside of the ''classical'' promoter region contributes to promoter activity.