An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach
An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach
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DOI：
10.1002/pmic.201000791
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发表时间：
2011-08-01
期刊：
影响因子：
3.4
通讯作者：
Noirot, Philippe
中科院分区：
文献类型：
--
作者：
Marchadier, Elodie;Carballido-Lopez, Rut;Noirot, Philippe
我们已经产生了一个蛋白质-蛋白质相互作用网络在枯草芽孢杆菌集中在几个基本的细胞过程，如细胞分裂，细胞对各种压力的反应，细菌的细胞骨架，DNA复制和染色体的维护，通过仔细应用酵母双杂交方法。该网络由793个相互作用组成，连接287个蛋白质，每个蛋白质平均连接5个相互作用，是未来功能分析的宝贵资源。该网络的一个显着特征是一组高度连接的枢纽（GoH）连接许多不同的细胞过程。GoH的大多数蛋白质具有未知的功能，并且与膜相关。通过整合现有的知识，特别是转录组数据集，GoH被分解为对应于蛋白质复合物或在不同条件下表达的调控途径的党枢纽的亚组。在全球范围内，GoH可能是一个非常强大的数据中心，具有部分冗余功能，可以整合来自不同细胞通路的信息。我们的分析也提供了一个合理的方式来研究的高度冗余功能的GoH的遗传方法。
We have generated a protein-protein interaction network in Bacillus subtilis focused on several essential cellular processes such as cell division, cell responses to various stresses, the bacterial cytoskeleton, DNA replication and chromosome maintenance by careful application of the yeast two-hybrid approach. This network, composed of 793 interactions linking 287 proteins with an average connectivity of five interactions per protein, represents a valuable resource for future functional analyses. A striking feature of the network is a group of highly connected hubs (GoH) linking many different cellular processes. Most of the proteins of the GoH have unknown functions and are associated to the membrane. By the integration of available knowledge, in particular of transcriptome data sets, the GoH was decomposed into subgroups of party hubs corresponding to protein complexes or regulatory pathways expressed under different conditions. At a global level, the GoH might function as a very robust group of date hubs having partially redundant functions to integrate information from the different cellular pathways. Our analyses also provide a rational way to study the highly redundant functions of the GoH by a genetic approach.