Insect bite hypersensitivity in the horse: Comparison of IgE-binding proteins in salivary gland extracts from Simulium vittatum and Culicoides nubeculosus
Insect bite hypersensitivity in the horse: Comparison of IgE-binding proteins in salivary gland extracts from Simulium vittatum and Culicoides nubeculosus
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DOI：
10.1016/j.vetimm.2009.09.016
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发表时间：
2009-11-15
影响因子：
1.8
通讯作者：
Marti, E.
中科院分区：
文献类型：
--
作者：
Hellberg, W.;Mellor, P. S.;Marti, E.
昆虫叮咬超敏反应(IBH)是一种由IgE介导的马过敏性皮炎，由库蚊属或类库蚊属等昆虫叮咬引起。本研究的目的是比较感染IBH的马血清与库蚊和牡蛎唾液腺提取物(SGE)的IgE结合模式。用免疫印迹法检测了15匹感染IBH的马和3匹健康马的血清免疫球蛋白E(IgE)反应。在15匹受IBH影响的马中，有14匹马的IgE分别与7条和6条主要蛋白条带结合，而健康马中没有一匹。这14份血清均能与两种沙门氏菌SGE蛋白结合，但与沙门氏菌SGE蛋白结合的条带较少。表现出免疫球蛋白E结合到32 kDa条带的血清总是结合到来自沙门氏菌的32 kDa条带。类似地，所有与核型隐翅虫70 kDa条带结合的血清都与链球菌SGE中的相应条带发生反应。经质谱鉴定为热休克蛋白-70同源蛋白-3。(C)2009爱思唯尔B.V.保留所有权利。
Insect bite hypersensitivity (IBH) is an IgE-mediated allergic dermatitis of horses caused by bites of insects such as Culicoides or Simulium spp. The aim of the present study was to compare the IgE-binding pattern of sera of IBH-affected horses to Culicoides nubeculosus and Simulium vittatum salivary gland extracts (SGE). Individual IgE responses to proteins of S. vittatum and C. nubeculosus SGEs were evaluated in 15 IBH-affected and three healthy horses on immunoblots. Fourteen out of the 15 IBH-affected but none of the healthy horses showed individual IgE binding patterns to seven and six main protein bands in C. nubeculosus and S. vittatum SGE, respectively. These 14 sera showed IgE-binding to proteins from SGE of both C. nubeculosus and S. vittatum, but they reacted with fewer protein bands derived from S. vittatum than from C. nubeculosus SGE. Sera showing IgE-binding to a 32 kDa band from C. nubeculosus always bound to a 32 kDa band from S. vittatum. Similarly, all sera binding to a 70 kDa band from C. nubeculosus reacted with a corresponding band in S. vittatum SGE. The 70 kDa bands from S. vittatum and C. nubeculosus were identified by mass spectrometry as heat shock protein-70-cognate-3. (C) 2009 Elsevier B.V. All rights reserved.