Transient alkalinization in the leaf apoplast of Vicia faba L. depends on NaCl stress intensity: an in situ ratio imaging study
Transient alkalinization in the leaf apoplast of Vicia faba L. depends on NaCl stress intensity: an in situ ratio imaging study
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DOI：
10.1111/j.1365-3040.2011.02437.x
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发表时间：
2012-03-01
影响因子：
7.3
通讯作者：
Muehling, Karl Hermann
中科院分区：
文献类型：
--
作者：
Geilfus, Christoph-Martin;Muehling, Karl Hermann
外质体不仅参与对环境信号的反应，还参与对各种环境信号的感知和转导。在此背景下，外胞体碱化已经作为非生物和生物应激事件引起的一般应激因素进行了讨论。本研究采用离子敏感荧光探针与倒置荧光显微镜相结合的方法，对蚕豆根系受到NaCl胁迫胁迫时，胞外芽的pH值进行了监测。结果表明，叶片外胞体pH值的短暂增加与NaCl胁迫强度有关。此外，我们还可视化了叶片外质体内的空间pH梯度。我们的研究结果表明，这些pH响应从根传播到叶，并沿着外质体发生。
The apoplast is suggested to be involved not only in the response, but also in the perception and transduction of various environmental signals. In this context, apoplastic alkalinization has previously been discussed as a general stress factor caused by abiotic and biotic stress events. In this study, an ion-sensitive fluorescence probe in combination with inverted fluorescence microscopy has been used for in planta monitoring of apoplastic shoot pH during challenging of Vicia faba L. plants by NaCl stress encountered at the roots. We demonstrate that transient increases in leaf apoplastic pH are dependent on the NaCl stress intensity. Moreover, we have visualized spatial pH gradients within the leaf apoplast. Our results indicate that these pH responses are propagated from root to leaf and that this occurs along the apoplast.