Embryonic cardiomyocyte, but not autologous stem cell transplantation, restricts infarct expansion, enhances ventricular function, and improves long-term survival.
Embryonic cardiomyocyte, but not autologous stem cell transplantation, restricts infarct expansion, enhances ventricular function, and improves long-term survival.
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DOI：
10.1371/journal.pone.0061510
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Strijkers GJ
中科院分区：
文献类型：
--
作者：
Paulis LE;Klein AM;Ghanem A;Geelen T;Coolen BF;Breitbach M;Zimmermann K;Nicolay K;Fleischmann BK;Roell W;Strijkers GJ
关于移植心脏或体细胞祖细胞对心肌损伤的有益作用存在争议。因此，我们研究了功能的短期和长期的后果后，这些细胞类型的心肌内移植在小鼠病变模型。在小鼠（n = 75）中诱导心肌梗死（MI），然后心肌内注射1 - 2×105个表达荧光素酶和GFP的胚胎心肌细胞（eCM）、骨骼肌成肌细胞（SM）、间充质干细胞（MSC）或培养基到梗死部位。 未处理的健康小鼠（n = 6）作为对照。 生物发光和荧光成像证实了细胞在术后长达七周的植入和存活。两周后进行MRI，结果显示仅使用eCM即可显着减少梗死体积（14.8± 2.2%MI +eCM vs. 26.7± 1.6%MI）。任何细胞类型的移植都能显著降低左心室扩张，但eCM最有效。此外，eCM治疗使射血分数和心输出量显著增加至33.4±2.2%和22.3±1.2 ml/min。此外，这种细胞类型专门显著增加梗死中心和边缘的收缩末期壁厚度，并增加梗死边缘的壁增厚。在以后的时间点重复超声心动图检查证实，这些有益的影响伴随着更好的生存率。与体细胞（SM和MSC）移植相比，采用收缩和电偶联胚胎心肌细胞（eCM）进入缺血心肌的细胞心肌成形术引起了显著较小的梗死，不良重塑较少，心脏功能和长期存活率改善，从而证明心肌细胞表型对恢复心肌功能很重要。
Controversy exists in regard to the beneficial effects of transplanting cardiac or somatic progenitor cells upon myocardial injury. We have therefore investigated the functional short- and long-term consequences after intramyocardial transplantation of these cell types in a murine lesion model. Myocardial infarction (MI) was induced in mice (n = 75), followed by the intramyocardial injection of 1−2×105 luciferase- and GFP-expressing embryonic cardiomyocytes (eCMs), skeletal myoblasts (SMs), mesenchymal stem cells (MSCs) or medium into the infarct. Non-treated healthy mice (n = 6) served as controls. Bioluminescence and fluorescence imaging confirmed the engraftment and survival of the cells up to seven weeks postoperatively. After two weeks MRI was performed, which showed that infarct volume was significantly decreased by eCMs only (14.8±2.2% MI+eCM vs. 26.7±1.6% MI). Left ventricular dilation was significantly decreased by transplantation of any cell type, but most efficiently by eCMs. Moreover, eCM treatment increased the ejection fraction and cardiac output significantly to 33.4±2.2% and 22.3±1.2 ml/min. In addition, this cell type exclusively and significantly increased the end-systolic wall thickness in the infarct center and borders and raised the wall thickening in the infarct borders. Repetitive echocardiography examinations at later time points confirmed that these beneficial effects were accompanied by better survival rates. Cellular cardiomyoplasty employing contractile and electrically coupling embryonic cardiomyocytes (eCMs) into ischemic myocardium provoked significantly smaller infarcts with less adverse remodeling and improved cardiac function and long-term survival compared to transplantation of somatic cells (SMs and MSCs), thereby proving that a cardiomyocyte phenotype is important to restore myocardial function.
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影响因子：
24
作者：
Li, Zongjin;Lee, Andrew;Huang, Mei;Chun, Hyung;Chung, Jaehoon;Chu, Pauline;Hoyt, Grant;Yang, Phillip;Rosenberg, Jarrett;Robbins, Robert C.;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
24
作者：
Hare, Joshua M.;Traverse, Jay H.;Henry, Timothy D.;Dib, Nabil;Strumpf, Robert K.;Schulman, Steven P.;Gerstenblith, Gary;DeMaria, Anthony N.;Denktas, Ali E.;Gammon, Roger S.;Hermiller, James B., Jr.;Reisman, Mark A.;Schaer, Gary L.;Sherman, Warren
通讯作者：
Sherman, Warren
影响因子：
20.1
作者：
Hatzistergos KE;Quevedo H;Oskouei BN;Hu Q;Feigenbaum GS;Margitich IS;Mazhari R;Boyle AJ;Zambrano JP;Rodriguez JE;Dulce R;Pattany PM;Valdes D;Revilla C;Heldman AW;McNiece I;Hare JM
通讯作者：
Hare JM
影响因子：
5.2
作者：
Li, Zongjin;Suzuki, Yoriyasu;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
37.8
作者：
Hill, JM;Dick, AJ;Lederman, RJ
通讯作者：
Lederman, RJ