ENHANCEMENT BY N-HYDROXYSULFOSUCCINIMIDE OF WATER-SOLUBLE CARBODIIMIDE-MEDIATED COUPLING REACTIONS
ENHANCEMENT BY N-HYDROXYSULFOSUCCINIMIDE OF WATER-SOLUBLE CARBODIIMIDE-MEDIATED COUPLING REACTIONS
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DOI：
10.1016/0003-2697(86)90176-4
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发表时间：
1986-07-01
影响因子：
2.9
通讯作者：
SWINGLE, DM
中科院分区：
文献类型：
--
作者：
STAROS, JV;WRIGHT, RW;SWINGLE, DM
水溶性碳二亚胺经常用于偶联或偶联反应，例如，将肽免疫原连接到载体蛋白上。然而，在某些条件下获得的低耦合产率限制了它们的应用。我们发现在这些反应中加入n -羟基磺基琥珀酰亚胺可以大大提高收率。
Water-soluble carbodiimides are frequently employed in coupling or conjugation reactions, e.g., to link a peptide immunogen to a carrier protein. However, their utility is limited by low coupling yields obtained under some conditions. We have found that addition of N-hydroxysulfosuccinimide to such reactions can greatly enhance the yields obtained.