Graded potential of neural crest to form cornea, sensory neurons and cartilage along the rostrocaudal axis
Graded potential of neural crest to form cornea, sensory neurons and cartilage along the rostrocaudal axis
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DOI：
10.1242/dev.01106
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发表时间：
2004-05-01
期刊：
影响因子：
4.6
通讯作者：
Bronner-Fraser, M
中科院分区：
文献类型：
--
作者：
Lwigale, PY;Conrad, GW;Bronner-Fraser, M
来自不同吻尾水平的神经脊细胞形成不同种类的衍生物，如神经节、软骨和角膜。这些差异可能是由于细胞群体的固有属性、迁移过程中遇到的不同环境因素或其某种组合造成的。我们通过将心脏和躯干神经脊细胞移植到异位中脑环境中来挑战心脏和躯干神经脊细胞的发育潜力，以测试内在因素和外在因素的相对作用。然后我们评估长期存活和分化成不同的衍生体，包括角膜、三叉神经节和颧弓软骨。尽管它们有能力迁移到眼周区域，但心脏或躯干神经脊对角膜的贡献都不适当，心脏脊细胞经常在角膜表面形成异位肿块。与对照中脑移植物相比，三叉神经节内主干和心脏神经脊形成体感神经元的潜力显著降低。心脏神经脊形成软骨的能力降低，仅对Meckle‘s软骨有名义贡献，而主干神经脊移植后没有形成软骨，即使直接移植到第一鳃弓上也不形成软骨。这些结果表明，即使在移植到允许的环境中后，沿吻尾轴的神经脊细胞仍显示出形成体感神经元和软骨的发育潜力的逐渐丧失。在移植到中脑后12小时，HOX基因在心脏神经管和神经脊中暂时保持表达，但随后下调。这表明，在这种情况下，HOX基因表达的长期差异不能解释这种情况下神经脊群发育潜力的吻尾差异。
Neural crest cells arising from different rostrocaudal axial levels form different sets of derivatives as diverse as ganglia, cartilage and cornea. These variations may be due to intrinsic properties of the cell populations, different environmental factors encountered during migration or some combination thereof. We test the relative roles of intrinsic versus extrinsic factors by challenging the developmental potential of cardiac and trunk neural crest cells via transplantation into an ectopic midbrain environment. We then assess long-term survival and differentiation into diverse derivatives, including cornea, trigeminal ganglion and branchial arch cartilage. Despite their ability to migrate to the periocular region, neither cardiac nor trunk neural crest contribute appropriately to the cornea, with cardiac crest cells often forming ectopic masses on the corneal surface. Similarly, the potential of trunk and cardiac neural crest to form somatosensory neurons in the trigeminal ganglion was significantly reduced compared with control midbrain grafts. Cardiac neural crest exhibited a reduced capacity to form cartilage, contributing only nominally to Meckle's cartilage, whereas trunk neural crest formed no cartilage after transplantation, even when grafted directly into the first branchial arch. These results suggest that neural crest cells along the rostrocaudal axis display a graded loss in developmental potential to form somatosensory neurons and cartilage even after transplantation to a permissive environment. Hox gene expression was transiently maintained in the cardiac neural tube and neural crest at 12 hours post-transplantation to the midbrain, but was subsequently downregulated. This suggests that long-term differences in Hox gene expression cannot account for rostrocaudal differences in developmental potential of neural crest populations in this case.