MicroRNA-128 promotes cell-cell adhesion in U87 glioma cells via regulation of EphB2
MicroRNA-128 promotes cell-cell adhesion in U87 glioma cells via regulation of EphB2
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MicroRNA-128 通过调节 EphB2 促进 U87 胶质瘤细胞的细胞间粘附
DOI：
10.3892/or.2013.2596
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发表时间：
2013-09-01
期刊：
影响因子：
4.2
通讯作者：
Jiang, Songshan
中科院分区：
文献类型：
--
作者：
Lin, Lina;Chen, Xulin;Jiang, Songshan
microRNA（miRNAs）是一类在转录后水平调控基因表达的小分子非编码RNA。miRNAs的异常表达经常发生在人类肿瘤中。尽管在神经胶质瘤组织和细胞中观察到miR-128的表达减少，但miR-128在肿瘤中的作用尚未完全表征。在本研究中，细胞粘附实验表明，miR-128的过表达可以促进细胞-细胞粘附。靶位点预测算法表明，miR-128结合促红细胞生成素肝细胞受体（Eph）B1和EphB 2 mRNA的3 '非翻译区。荧光素酶报告基因分析证实miR-128结合并调节EphB 1和EphB 2 mRNA。EphB 2的过表达降低了miR-128促进细胞-细胞粘附的能力。伤口愈合试验表明，miR-128通过EphB 2显著抑制细胞迁移。本研究揭示了miR-128通过调控EphB 2在胶质瘤细胞中的细胞间粘附和细胞迁移中的新功能，并将EphB 1和EphB 2确定为miR-128的新靶点。
MicroRNAs (miRNAs) are small, non-coding RNAs which regulate gene expression at the post-transcriptional level. Abnormal expression of miRNAs occurs frequently in human tumors. Despite the fact that reduced expression of miR-128 has been observed in glioma tissues and cells, the role of miR-128 in tumors has not been fully characterized. In the present study, cell adhesion assays indicated that overexpression of miR-128 can promote cell-cell adhesion. Target site prediction algorithms indicated that miR-128 binds the 3'-untranslated regions of erythropoietin-producing hepatocellular receptor (Eph)B1 and EphB2 mRNAs. Luciferase reporter assays confirmed that miR-128 binds and regulates EphB1 and EphB2 mRNAs. Overexpression of EphB2 reduced the ability of miR-128 to promote cell-cell adhesion. The wound-healing assay indicated that miR-128 significantly inhibited cell migration via EphB2. This study revealed the novel functions of miR-128 in cell-cell adhesion and cell migration in glioma cells through the regulation of EphB2, and identified EphB1 and EphB2 as novel miR-128 targets.