EFFECTS OF PH, LACTATE, AND VISCOELASTIC DRAG ON SPERM MOTILITY - A SPECIES COMPARISON
EFFECTS OF PH, LACTATE, AND VISCOELASTIC DRAG ON SPERM MOTILITY - A SPECIES COMPARISON
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DOI：
10.1095/biolreprod33.3.588
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发表时间：
1985-01-01
影响因子：
3.6
通讯作者：
ACOTT, TS
中科院分区：
文献类型：
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作者：
CARR, DW;USSELMAN, MC;ACOTT, TS
5种哺乳动物的精子在附睾尾液(CEF)中体外孵育时，精子的活动度很低或没有。我们研究了pH、乳酸和粘弹性阻力对精子活力的影响，以确定这些因素是否与精子活力的抑制有关。牛、狗、大鼠、豚鼠和仓鼠CEF的PHS分别为5.8、6.2、6.9、6.9和7.2。从麻醉动物采集的附睾精液乳酸浓度在0.6到0.9之间，但在大鼠或狗的样本中，在死后2小时测量时，乳酸浓度几乎增加了10倍。将CEF的pH值提高到7.0，可使牛、狗精子完全运动。将精子悬浮在不同pH值(从4.0到7.6)的缓冲液中，所有物种的精子运动曲线均为S型。所有物种，包括公牛和狗，在pH等于其自身CEF的pH时，在缓冲液中几乎完全活动。将牛、狗精子悬浮在CEF中而不是缓冲液中，随着pH值的降低，牛、狗精子的活力受到较大的抑制。添加15 mM乳酸可以降低精子细胞内的pH，使所有物种的精子活力-pH曲线向更高的pH方向移动。在公牛和狗身上，添加乳酸产生了一种与它们自己的CEF中没有区别的运动图谱。只有两个物种的CEF的粘弹性阻力足够高，足以抑制精子的运动。我们的结论是，公牛和狗的CEF的低pH加上乳酸的存在足以导致运动抑制。大鼠和仓鼠精子活力抑制可以用它们的CEF的高粘弹性阻力来解释。豚鼠CEF具有较高的pH和相对较低的阻力，可能受到其他机制的抑制。
Little or no motility is observed when sperm from 5 mammalian species are incubated in vitro in their cauda epididymal fluid (CEF). We examined the effects of pH, lactate, and viscoelastic drag on sperm motility to determine whether these factors are responsible for this inhibition of motility. The pHs of CEF from bull, dog, rat, guinea pig, and hamster were 5.8, 6.2, 6.9, 6.9, and 7.2, respectively. The lactate concentration of epididymal semen collected from anesthetized animals ranged from 0.6 to 0.9, but increased almost 10-fold in samples from rats or dogs when measured 2 h postmortem. Increasing the pH of CEF to 7.0 resulted in the initiation of full motility for bull and dog sperm. Suspensions of sperm in buffer at various pHs (from 4.0 to 7.6) produced a sigmoidal motility curve for all species. All species, including bull and dog, showed almost full motility in buffer at a pH equal to the pH of their own CEF. Motility of bull and dog sperm showed greater inhibition with decreasing pH when suspended in CEF instead of buffer. The addition of 15 mM lactate, which has been shown to lower sperm intracellular pH, shifted the motility versus pH curves of all species toward higher pH. In bull and dog the addition of lactate produced a motility profile that was indistinguishable from that in their own CEF. The viscoelastic drag of the CEF of only two species, rat and hamster, was sufficiently high to inhibit sperm motility. We conclude that the low pH of the CEF from bulls and dogs plus the presence of lactate is sufficient to cause inhibition of motility. Rat and hamster sperm motility inhibition can be explained by the high viscoelastic drag of their CEF. Guinea pig CEF, which has a high pH and a relatively low drag, may inhibit by some other mechanism.