Reduced Susceptibility to Cefepime in Clinical Isolates of Enterobacteriaceae Producing OXA-1 Beta-Lactamase.
Reduced Susceptibility to Cefepime in Clinical Isolates of Enterobacteriaceae Producing OXA-1 Beta-Lactamase.
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产生 OXA-1 β-内酰胺酶的肠杆菌科临床分离株对头孢吡肟的敏感性降低。
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
PascualÁlvaro
中科院分区：
文献类型：
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作者：
TorresEva;López;Rodríguez;PascualÁlvaro
本地区对头孢吡肟（FEP）和阿莫西林/克拉维酸酯（AMC）敏感性降低的肠杆菌科分离株有所增加。本研究的目的是表征这种抗生素耐药表型及其分子流行病学。共研究了33株肠杆菌科菌株。采用聚合酶链反应（PCR）和测序技术对blaOXA-1基因及其遗传环境进行分析。质粒通过偶联和/或转化转移，并使用基于pcr的inc/rep分型和IncF分型进行分类。采用系统群、脉冲场凝胶电泳（PFGE）和多位点序列分型对分离的大肠杆菌进行分型。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和逆转录- pcr法检测blaOXA-1基因的表达。FEP最低抑菌浓度为1 ~ 16mg /L。产生OXA-1的菌株29株（87.9%），其中24株（82.7%）位于1类整合子；
An increase of Enterobacteriaceae isolates with reduced susceptibility to cefepime (FEP) and amoxicillin/clavulanate (AMC) has been observed in our area. The aim of this study was to characterize this antibiotic resistance phenotype and its molecular epidemiology. A total of 33 Enterobacteriaceae strains were studied. blaOXA-1 genes and their genetic environment were analyzed by polymerase chain reaction (PCR) and sequencing. Plasmids were transferred by conjugation and/or transformation and classified using PCR-based inc/rep typing and IncF subtyping. Escherichia coli isolates were typed by phylogroup, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. Outer membrane proteins were studied by sodium dodecylsulfate–polyacrylamide gel electrophoresis and expression of blaOXA-1 genes by reverse transcription-PCR. FEP minimum inhibitory concentration yielded values of 1–16 mg/L. Twenty-nine (87.9%) isolates produced OXA-1, of which 24 (82.7%) were located in class 1 integron, and 9 (27.3%...