Elite sd1 alleles in japonica rice and their breeding applications in northeast China
Elite sd1 alleles in japonica rice and their breeding applications in northeast China
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粳稻优良sd1等位基因及其在东北育种中的应用
DOI：
10.1016/j.cj.2021.05.005
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发表时间：
2021-06
期刊：
影响因子：
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通讯作者：
Jun Fang
中科院分区：
文献类型：
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作者：
Hanjing Sha;Hualong Liu;Guangxin Zhao;Zhongmin Han;Huilin Chang;Jingguo Wang;Hongliang Zheng;Jifeng Zhang;Yang Yu;Yuqiang Liu;Detang Zou;Shoujun Nie;Jun Fang
绿色革命基因1已广泛应用于现代水稻育种，特别是在水稻品种上。然而，用于粳稻育种的优良等位基因和相关种质资源尚未鉴定出来，粳稻育种还需要进行广泛的努力，以获得新的矮源。MBKbase-Rice的数据显示，在籼稻和粳稻中分别有7个和4个单倍型。在籼稻中发现2个news d1等位基因。在295份来自东北亚的粳稻种质中，除弱功能等位基因SD 1-EQ外，sd 1-r为主要等位基因，与SD 1-EQ相比，sd 1-r降低株高。家系和地理分布表明，sd 1-是一个广泛应用于中国北方和日本东北地区的优良等位基因，sd 1-j在中国东部和日本九州地区广泛使用。东农系列和秀水系列品种分别携带d1-randsd 1-j，是粳稻、秋光和测21骨干亲本的基本分支，后代和衍生世代数最多。在半矮秆粳稻育种中，将sd 1-d基因导入稻花香2号（DHX 2）。在2020年经受台风引发的大风和暴雨的考验后，选择了携带d1-d的矮秆和半矮秆系，分别命名为1279和1280，预计将成为未来遗传研究和育种的有用中间材料。本研究将为引种、亲本选择和分子标记辅助育种提供便利，并为下一步鉴定与这些d1等位基因共同选择的有利基因提供物质基础。
The Green Revolution genesd1has been used extensively in modern rice breeding, especially inindicacultivars. However, elitesd1alleles and related germplasm resources used forjaponicarice breeding have not been identified, and extensive efforts are needed forjaponicarice breeding to obtain new dwarfing sources. Data from MBKbase-Rice revealed sevensd1haplotypes inindicaand four injaponicarice. Two newsd1alleles were identified inindicarice. In 295japonicaaccessions from northeast Asia, except for the weak functional alleleSD1-EQ,sd1-rwas the major allele, reducing plant height in comparison withSD1-EQ.Japonicagermplasm resources carrying reportedsd1alleles were identified by genotype searching and further verified by literature search, genealogical analysis, and dCaps markers.Pedigrees and geographic distribution showed thatsd1-ris an excellent allele widely used in northern China and Tohoku in Japan, andsd1-jis commonly used in east China and Kyushu in Japan. Dongnong- and Xiushui-series cultivars carryingsd1-randsd1-j, respectively, are essential branches of the backbone parents of Chinesejaponicarice, Akihikari and Ce21, with the largest number of descendants and derived generations. In semi-dwarfjaponicarice breeding,sd1-dwas introgressed into Daohuaxiang 2 (DHX2). Dwarf and semi-dwarf lines carryingsd1-dwere selected and designated as 1279 and 1280, respectively, after withstanding typhoon-induced strong winds and heavy rains in 2020, and are anticipated to become useful intermediate materials for future genetic research and breeding. This work will facilitate the introduction, parental selection, and marker-assisted breeding, and provide a material basis for the next step in identifying favorable genes that selected together with thesd1alleles injaponicabackbone parents.