miR-30a inhibits glioma progression and stem cell-like properties by repression of Wnt5a
miR-30a inhibits glioma progression and stem cell-like properties by repression of Wnt5a
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DOI：
10.3892/or.2017.5728
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发表时间：
2017-08-01
期刊：
影响因子：
4.2
通讯作者：
Xie, Min
中科院分区：
文献类型：
--
作者：
Zhang, Yonghong;Wu, Zhi;Xie, Min
已经发现miR-30 a在多种癌症中失调，并参与肿瘤进展的调节。然而，关于miR-30 a在胶质瘤中的作用的研究却很少。在本研究中，我们评估了miR-30 a在胶质瘤组织和细胞系中的表达水平。microRNA微阵列分析显示miR-30 a在胶质瘤组织和细胞中的表达低于对照组。此外，我们发现稳定的miR-30 a抑制胶质瘤细胞增殖，G（1）期阻滞和干细胞样形成。此外，为了探讨miR-30 a对胶质瘤细胞表型的影响机制，我们通过生物信息学分析、荧光素酶分析和western blot分析确定了Wnt 5a为miR-30 a的新的直接靶基因。进一步的功能研究表明，miR-30 a通过靶向Wnt 5a信号通路抑制肿瘤转移、球体形成和胶质瘤生长。总的来说，我们的研究结果首次表明，miR-30 a可能通过靶向Wnt 5a在胶质瘤中发挥肿瘤抑制剂的作用。
miR-30a has been found to be dysregulated in diverse cancers and involved in the regulation of tumor progression. However, there is scarce research on the role of miR-30a in glioma. In the present study, we assessed the expression level of miR-30a in glioma tissues and cell lines. The microRNA microarray analysis revealed low expression of miR-30a in glioma tissues and cells vs. the control. Furthermore, we found that stable miR-30a inhibited cell proliferation, G(1) phase arrest and stem cell-like formation in glioma. Moreover, to investigate the molecular mechanism of miR-30a on glioma cell phenotypes, we identified Wnt5a as a new direct target gene for miR-30a by bioinformatic assay, luciferase assay and western blot analysis. Further functional studies suggested that miR-30a suppressed metastasis, sphere formation and glioma growth by targeting Wnt5a signal pathway. Collectively, our findings suggested for the first time that miR-30a may function as a tumor suppressor in glioma by targeting Wnt5a.