Defective initiation in an Escherichia coli dnaA(Cs,Sx) mutant
Defective initiation in an Escherichia coli dnaA(Cs,Sx) mutant
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DOI：
10.1016/s0300-9084(00)01230-x
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发表时间：
2001-01-01
期刊：
影响因子：
3.9
通讯作者：
Walker, JR
中科院分区：
文献类型：
--
作者：
Boye, E;Blinkova, A;Walker, JR
大肠杆菌DNA复制起始基因dnaA中的突变称为Cs，Sx，其抑制聚合基因dnaX中的温度敏感性（Ts）突变引起的聚合缺陷和生长抑制。我们在这里表明，这些突变本身也会导致启动缺陷，包括在低温（20 ℃）和高温（44 ℃）下的启动抑制，以及在允许温度（34 ℃）和抑制温度（39 ℃）下的异步启动。这些发现表明部分缺陷引发和抑制聚合缺陷之间的关系。两者都发生在39摄氏度。(C)2001法国生物化学与生物分子学会/科学与医学版Elsevier SAS。
Mutations in the Escherichia coli gene for initiation of DNA replication, dnaA, which suppress the polymerization defect and growth inhibition caused by temperature-sensitive (Ts) mutations in the polymerization gene, dnaX, are called Cs,Sx. We show here that these mutations, on their own, also cause defects in initiation, including inhibition of initiation at both low (20 degreesC) and high (44 degreesC) temperatures and asynchronous initiation at both the permissive (34 degreesC) and suppression (39 degreesC) temperatures. These findings suggests a relationship between partially defective initiation and suppression of the polymerization defect. both of which occur at 39 degreesC. (C) 2001 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS.