STUDIES ON OXIDATION AND REDUCTION BY PNEUMOCOCCUS : III. REDUCTION OF METHYLENE BLUE BY STERILE EXTRACTS OF PNEUMOCOCCUS.
STUDIES ON OXIDATION AND REDUCTION BY PNEUMOCOCCUS : III. REDUCTION OF METHYLENE BLUE BY STERILE EXTRACTS OF PNEUMOCOCCUS.
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DOI：
10.1084/jem.39.4.543
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发表时间：
1924-03-31
期刊：
影响因子：
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通讯作者：
Neill JM
中科院分区：
文献类型：
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作者：
Avery OT;Neill JM
1. 未洗涤的肺炎球菌的无菌肉汤提取物，完全不含活细胞或完整细胞，可主动还原亚甲蓝。 2. 在磷酸盐溶液中提取洗涤的肺炎球菌而制备的无菌提取物本身不能还原亚甲蓝。添加肉浸液或酵母提取物后，这些经过洗涤的细胞提取物会主动还原亚甲蓝。 3.肺炎球菌提取物中负责亚甲蓝还原的一个或多个系统通过暴露于与先前发现的破坏相同提取物的过氧化物形成活性的温度几乎相同的温度而被破坏。 4.有人认为，肺炎球菌提取物的过氧化物形成和亚甲蓝还原作用是相同或密切相关的系统的功能，具体反应取决于分子氧或亚甲蓝是否充当氢受体或氧供体。
1. Sterile broth extracts of unwashed pneumococci, entirely free from living or intact cells, actively reduce methylene blue. 2. Sterile extracts prepared by extracting washed pneumococci in phosphate solution are unable by themselves to reduce methylene blue. Upon the addition of meat infusion or yeast extract, these washed cell extracts actively reduce methylene blue. 3. The system or systems in pneumococcus extracts responsible for methylene blue reduction are destroyed by exposure to temperatures practically identical with those which have been previously found to destroy the peroxide-forming activity of the same extracts. 4. It is suggested that peroxide formation and methylene blue reduction by pneumococcus extracts are functions of the same or closely related systems, the particular reaction induced depending upon whether molecular oxygen or methylene blue serves as hydrogen acceptor or oxygen donator.