Nattokinase Crude Extract Enhances Cutaneous Wound Healing
Nattokinase Crude Extract Enhances Cutaneous Wound Healing
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纳豆激酶粗提取物促进皮肤伤口愈合
DOI：
10.1166/jbt.2017.1697
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发表时间：
2017-12
影响因子：
0.1
通讯作者：
Gong Aihua
中科院分区：
文献类型：
--
作者：
Zhang Bin;Liu Yuwan;Ji Qian;Zhao Mengmeng;Zeng Jian;Liu Lihua;Xu Xiao;Yan Yongmin;Gong Aihua
纳豆激酶（NK）是一种由枯草芽孢杆菌产生的纤溶酶。这种酶来自于传统的日本发酵食品纳豆，对多种疾病有治疗作用，包括高血压、阿尔茨海默病和玻璃体视网膜疾病。NK在皮肤组织再生中是否有效尚不清楚。利用超滤膜将枯草芽孢杆菌培养基分离成不同分子量的样品。在枯草芽孢杆菌培养基中，只有分子量为10 kDa (K) ~ 100 K的组分具有NK活性；将这些成分命名为纳豆激酶粗提物（NCE）。NCE在小鼠皮肤切除损伤中表现出促进伤口愈合的能力。与培养上清液中的其他成分相比，NCE对表皮再生和血管生成的影响更为明显。nce处理组皮肤组织中Oct-4和Nanog的表达水平显著升高。本研究揭示了NK在皮肤创面愈合中的新作用，并证明NCE扩展了NK的应用。
Nattokinase (NK) is a fibrinolytic enzyme produced by Bacillus subtilis. This enzyme comes from a traditional Japanese fermented food called natto, and has therapeutic effects in a variety of diseases including hypertension, Alzheimer's disease, and vitreoretinal disorders. Whether NK is effective in skin tissue regeneration is unclear. Using ultrafiltration membranes, we separated a cultured medium of Bacillus subtilis into samples of different molecular weights. Of all the components from the cultured medium of Bacillus subtilis, only the 10 kDa (K) to 100 K molecular weight components had NK activity; these components were named nattokinase crude extract (NCE). NCE demonstrated the ability to promote wound healing in mouse skin-resection injuries. Relative to the other ingredients in the cultured supernatant, NCE has a more pronounced effect on epidermal regeneration and angiogenesis. The expression levels of Oct-4 and Nanog in the skin tissue of the NCE-treated group were significantly higher. This study reveals a new role for NK in cutaneous wound healing and demonstrates that NCE extends the application of NK.