The osmoregulatory effects of rearing Mozambique tilapia in a tidally changing salinity
The osmoregulatory effects of rearing Mozambique tilapia in a tidally changing salinity
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DOI：
10.1016/j.ygcen.2014.03.013
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发表时间：
2014-10-01
影响因子：
2.7
通讯作者：
Seale, Andre P.
中科院分区：
文献类型：
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作者：
Moorman, Benjamin P.;Inokuchi, Mayu;Seale, Andre P.
莫桑比克罗非鱼 Oreochromis mossambicus 的原生分布特点是河口地区受淡水 (FW) 和海水 (SW) 盐度随潮汐频率变化的影响。面对环境盐度变化的渗透调节主要通过神经内分泌系统介导，并涉及渗透调节组织中离子摄取和排出机制的激活。我们比较了在 FW、SW、咸水 (BW) 和潮汐变化盐度中饲养的罗非鱼的血浆渗透压、血浆催乳素 (PRL)、垂体 PRL mRNA 以及鳃离子泵、转运蛋白、通道和 PRL 受体的 mRNA，盐度每 6 小时在 FW (TF) 和 SW (TS) 之间变化。 FW 罗非鱼的血浆 PRL 高于 SW、BW、TF 和 TS 罗非鱼。与在FW或SW中饲养的罗非鱼不同，在潮汐变化的盐度中的鱼显示血浆渗透压和PRL之间没有相关性。在FW鱼中，PRL受体1（PRLR1）、Na+/Cl-协同转运蛋白（NCC）、水通道蛋白3（AQP3）和Na+/K+-ATP酶的两种亚型（NKA α 1a和NKA α 1b）的基因表达高于SW、BW或潮汐变化盐度鱼。 Na+/K+/2Cl(-)协同转运蛋白(NKCC1a)和囊性纤维化跨膜电导调节因子(CFTR)的基因表达在SW、BW或潮汐变化盐度条件下的鱼中高于FW鱼。免疫细胞化学显示，潮汐变化盐度下的鱼类离子细胞与西南鱼类的离子细胞相似。这项研究表明，在潮汐变化的盐度中饲养的罗非鱼可以补偿外部渗透压的巨大变化，同时将渗透调节参数保持在更接近于西南适应鱼中观察到的狭窄范围内。 (C) 2014 Elsevier Inc. 保留所有权利。
The native distribution of Mozambique tilapia, Oreochromis mossambicus, is characterized by estuarine areas subject to salinity variations between fresh water (FW) and seawater (SW) with tidal frequency. Osmoregulation in the face of changing environmental salinity is largely mediated through the neuroendocrine system and involves the activation of ion uptake and extrusion mechanisms in osmoregulatory tissues. We compared plasma osmolality, plasma prolactin (PRL), pituitary PRL mRNA, and mRNA of branchial ion pumps, transporters, channels, and PRL receptors in tilapia reared in FW, SW, brackish water (BW) and in tidally-changing salinity, which varied between FW (TF) and SW (TS) every 6 h. Plasma PRL was higher in FW tilapia than in SW, BW, TF, and TS tilapia. Unlike tilapia reared in FW or SW, fish in salinities that varied tidally showed no correlation between plasma osmolality and PRL. In FW fish, gene expression of PRL receptor 1 (PRLR1), Na+/Cl- cotransporter (NCC), aquaporin 3 (AQP3) and two iso-forms of Na+/K+-ATPase (NKA alpha 1a and NKA alpha 1b) was higher than that of SW, BW or tidally-changing salinity fish. Gene expression of the Na+/K+/2Cl(-) cotransporter (NKCC1a), and the cystic fibrosis transmembrane conductance regulator (CFTR) were higher in fish in SW, BW or a tidally-changing salinity than in FW fish. Immunocytochemistry revealed that ionocytes of fish in tidally-changing salinities resemble ionocytes of SW fish. This study indicated that tilapia reared in a tidally-changing salinity can compensate for large changes in external osmolality while maintaining osmoregulatory parameters within a narrow range closer to that observed in SW-acclimated fish. (C) 2014 Elsevier Inc. All rights reserved.