Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation
Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation
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DOI：
10.1007/s12010-012-9542-x
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发表时间：
2012-03-01
影响因子：
3
通讯作者：
Liu, Chun-Zhao
中科院分区：
文献类型：
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作者：
Zhao, Yan;Sun, Wei;Liu, Chun-Zhao
采用浸没式生物反应器系统对大花红景天进行快速增殖。在每300 min浸泡3 min的间歇浸泡周期中，芽增殖率和超水化率分别达到46.8和35.4%。在间歇浸泡生物反应器培养中采用强制通风，以降低超水化率，改善芽质量，提高增殖率。在空气流量为40 l/h的强制通风条件下，每180 min浸泡3 min的暂时浸泡周期获得最高增殖率55.7，过度水合率降低至26.1%。强制通风也提高了随后的伸长和生根率，这些增殖的芽，和芽培养物从暂时浸泡生物反应器形成完整的植株时，继代培养到生根培养基中含有5 μ mol/L吲哚-3-乙酸。
A temporary immersion bioreactor system was found to be suitable for mass shoot proliferation of Rhodiola crenulata. The shoot multiplication ratio and hyperhydration rate reached 46.8 and 35.4%, respectively, at a temporary immersion cycle of 3-min immersion every 300 min. Forced ventilation was employed in the temporary immersion bioreactor culture in order to decrease the hyperhydration rate, improve shoot quality and enhance the multiplication ratio. The highest multiplication ratio of 55.7 was obtained under a temporary immersion cycle of 3-min immersion every 180 min with the forced ventilation at an air flow rate of 40 l/h, and the hyperhydration rate was reduced to 26.1%. Forced ventilation also improved the subsequent elongation and rooting rate of these proliferated shoots, and the shoot cultures from the temporary immersion bioreactor formed complete plantlets when subcultured onto a rooting medium containing 5 mu mol/l indole-3-acetic acid.