Microsatellite Analysis of Genetic Diversity in Pekin (Anas platyrhynchos) and Muscovy (Cairina moschata) Duck Populations
Microsatellite Analysis of Genetic Diversity in Pekin (Anas platyrhynchos) and Muscovy (Cairina moschata) Duck Populations
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DOI：
10.3923/ijps.2007.378.382
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发表时间：
2007-05
期刊：
影响因子：
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通讯作者：
A. Ahmadi;G. Rahimi;A. Vafaei;H. Sayyazadeh
中科院分区：
文献类型：
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作者：
A. Ahmadi;G. Rahimi;A. Vafaei;H. Sayyazadeh
本研究利用13个微卫星标记对伊朗北部马赞达兰省北京鸭和莫斯科鸭群体的遗传结构进行了分析。每个群体各取100份血样，采用改良盐析法提取DNA。PCR扩增产物经6%聚丙烯酰胺凝胶电泳后，进行凝胶电泳。12个微卫星标记中有4个在两个群体中均未扩增，3个标记为单态，6个标记产生多态性条带。估计了两个鸭群体间的观察等位基因数、有效等位基因数、平均杂合度和遗传距离等遗传参数。每个位点的观察等位基因数为1-4，有效等位基因数为1 ~ 3.78，杂合度为0 ~ 0.98，两群体间的遗传距离为0.59个百分点。两种群间的遗传距离较低，平均杂合度指数较低，表明种群内和种群间的遗传多样性较低。低的平均杂合度可能是由于低数量的等位基因存在于人口中，高水平的近亲繁殖值，因为小的有效人口规模所涉及的研究鸭育种群。Hardy-Weinberg平衡检验结果表明，各微卫星位点均未达到平衡。本研究结果表明，利用分子生物学手段对鸭的遗传多样性进行表征是制定鸭遗传资源保护和利用策略的前提。
In the present study the genetic structure of Pekin and Moscovy duck populations in north of Iran, Mazandaran province were analyzed using thirteen microsatellite markers. One hundred blood samples with equal number from each population were collected and DNA was extracted using modified salting out method. After Polymerase Chain Reaction (PCR), the PCR products were electrophoresed using 6% polyacrylamide gel. Four Out of 12 microsatellite markers were not amplified in any of both populations, three markers were monomorph and six markers generated polymorph bands. Some genetic parameters such as observed and effective allele number, mean of heterozygosity and genetic distance between two duck populations were estimated. The observed allele number in each locus was ranged from 1-4, effective allele number from 1-3.78, heterozygosity from 0-0.98 and the genetic distance between two populations was measured as 0.59 percentages. The low of genetic distance between two populations and the low level of mean heterozygosity index indicate that the genetic diversity is low in within and between populations. The low mean heterozygosity may be attributed to the low number of alleles present in the population, high level of inbreeding values because of small effective population size involved in studied duck breeding flocks. The analysis of Hardy-Weinberg equilibrium test showed that none of the microsattelite sites were at equilibrium. The obtained results at the present study indicated that characterization of genetic diversity by employing molecular tools is a prerequisite in developing strategies for conservation and utilization of duck genetic resources.