Brief Report: Self-Organizing Neuroepithelium from Human Pluripotent Stem Cells Facilitates Derivation of Photoreceptors
Brief Report: Self-Organizing Neuroepithelium from Human Pluripotent Stem Cells Facilitates Derivation of Photoreceptors
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DOI：
10.1002/stem.1268
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发表时间：
2013-02-01
期刊：
影响因子：
5.2
通讯作者：
Ali, Robin R.
中科院分区：
文献类型：
--
作者：
Boucherie, Cedric;Mukherjee, Sayandip;Ali, Robin R.
视网膜色素变性，其他遗传性视网膜疾病，以及年龄相关性黄斑变性，由于光感受器的丧失而导致无法治疗的失明。我们最近已经证明，移植小鼠光感受器可以改善视力。因此，开发有效地从人类多能干细胞(HPS)中获得光感受器的方案是及时的。目前从HPS细胞获得光感受器的方法需要长时间的培养，并且效率相当低。在这里，我们报道了由人胚胎干细胞与细胞外基质一起培养形成的瞬时自组织神经上皮足以在5天内诱导快速转化为视网膜前体细胞。这些视网膜前体细胞仅在10天后就能非常有效地分化为CRX(+)光感受器前体，随后在4周内获得杆状光感受器特性。使用这种方法定向分化为光感受器也可以用人类诱导的多能干细胞(HIPS)细胞，促进将患者特定的HIPS细胞系用于再生医学和疾病建模。干细胞2013；31：408-414
Retinitis pigmentosa, other inherited retinal diseases, and age-related macular degeneration lead to untreatable blindness because of the loss of photoreceptors. We have recently shown that transplantation of mouse photoreceptors can result in improved vision. It is therefore timely to develop protocols for efficient derivation of photoreceptors from human pluripotent stem (hPS) cells. Current methods for photoreceptor derivation from hPS cells require long periods of culture and are rather inefficient. Here, we report that formation of a transient self-organized neuroepithelium from human embryonic stem cells cultured together with extracellular matrix is sufficient to induce a rapid conversion into retinal progenitors in 5 days. These retinal progenitors have the ability to differentiate very efficiently into Crx(+) photoreceptor precursors after only 10 days and subsequently acquire rod photoreceptor identity within 4 weeks. Directed differentiation into photoreceptors using this protocol is also possible with human-induced pluripotent stem (hiPS) cells, facilitating the use of patient-specific hiPS cell lines for regenerative medicine and disease modeling. STEM CELLS 2013;31:408-414