Neuropilin is a receptor for the axonal chemorepellent Semaphorin III
Neuropilin is a receptor for the axonal chemorepellent Semaphorin III
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DOI：
10.1016/s0092-8674(00)80534-6
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发表时间：
1997-08-22
期刊：
影响因子：
64.5
通讯作者：
TessierLavigne, M
中科院分区：
文献类型：
--
作者：
He, ZG;TessierLavigne, M
在发育中的神经系统中，延伸的轴突通过吸引和排斥的引导线索的协调动作被引导到它们的目标。脑信号蛋白家族的指导线索包括几个成员，可以作为扩散性轴突化学排斥。为了开始阐明Collapsin-1/Semaphorin III/D（Sema III）介导排斥作用的机制，我们通过表达克隆在胚胎大鼠感觉神经元中寻找Sema III结合蛋白。我们报告说，Sema III结合具有高亲和力的跨膜蛋白neuropilin，和抗体neuropilin块的能力，Sema III排斥感觉轴突，并诱导其生长锥崩溃。这些结果提供了证据表明，neuropilin是一种受体或受体复合物的一个组成部分，介导的影响，Sema III对这些轴突。
Extending axons in the developing nervous system are guided to their targets through the coordinate actions of attractive and repulsive guidance cues. The semaphorin family of guidance cues comprises several members that can function as diffusible axonal chemorepellents. To begin to elucidate the mechanisms that mediate the repulsive actions of Collapsin-1/Semaphorin III/D (Sema III), we searched for Sema III-binding proteins in embryonic rat sensory neurons by expression cloning. We report that Sema III binds with high affinity to the transmembrane protein neuropilin, and that antibodies to neuropilin block the ability of Sema III to repel sensory axons and to induce collapse of their growth cones. These results provide evidence that neuropilin is a receptor or a component of a receptor complex that mediates the effects of Sema III on these axons.