Parental contribution and growth hormone gene polymorphism associated with growth phenotypes of red sea bream Pagrus major in mass production: a case study.
Parental contribution and growth hormone gene polymorphism associated with growth phenotypes of red sea bream Pagrus major in mass production: a case study.
复制标题
亲本贡献和生长激素基因多态性与大规模生产真鲷生长表型相关：案例研究。
DOI：
10.1016/j.aqrep.2015.10.001
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发表时间：
2015
影响因子：
3.7
通讯作者：
Motohiro Takagi
中科院分区：
文献类型：
--
作者：
Eitaro Sawayama;Motohiro Takagi
红鲷鱼是日本最重要的养殖鱼种之一。为了提高该鱼在种子生产中的生产力，需要改善生长性状和减小尺寸变异。在本研究中，我们评估了在两个不同饲养阶段（1)在水箱中饲养50 dph和(2)在网笼中饲养200 dph）中观察到的快速和缓慢生长个体的亲代贡献。我们还基于先前开发的微卫星DNA标记评估了生长激素基因（pmaGH）多态性。在50和200 dph时，特定的亲鱼个体与快速或缓慢生长的个体显著相关。在50 dph条件下，快速生长组和缓慢生长组的pmaGH-740和pmagh -900等位基因频率存在显著差异。结合DNA亲本分析和pmagh -740微卫星等位基因分析结果，1株和2株pmagh -740等位基因与慢生长群体显著相关。这些结果表明，pmagh的小卫星标记可以作为该鱼类生长选择的有用工具。
Red sea bream is one of the most important aquaculture fish species in Japan. To improve the productivity of this fish during seed production, improved growth traits and reduced size variation are needed. In this study, we assessed parental contribution of fast- and slow-growing individuals observed in two different rearing phases in a mass production lot: (1) 50 dph reared in a tank and (2) 200 dph reared in a net cage. We also assessed GH gene (pmaGH) polymorphisms based on a previously developed minisatellite DNA marker. Specific broodstock individuals were significantly associated with fast- or slow-growing individuals at 50 dph and 200 dph. Significant differences inpmaGHminisatellite allele frequencies were observed between fast- and slow-growing groups at 50 dph in the frequency of two alleles (pmaGH-740 andpmaGH-900, respectively). Combining the results of DNA parentage analysis andpmaGHminisatellite allele analysis, one dam and two sires, possessingpmaGH-740, were significantly associated with the slow-growing groups. These results suggest that the minisatellite marker ofpmaGHcould be a useful tool for growth selection of this fish species.