Effect of tibial nerve stimulation on bladder afferent nerve activity in a rat detrusor overactivity model
Effect of tibial nerve stimulation on bladder afferent nerve activity in a rat detrusor overactivity model
复制标题
DOI：
10.1111/iju.13033
复制
发表时间：
2016-03-01
影响因子：
2.6
通讯作者：
van Asselt, Els
中科院分区：
文献类型：
--
作者：
Choudhary, Mahipal;van Mastrigt, Ron;van Asselt, Els
目的 研究胫神经刺激后对大鼠膀胱传入活动的影响，以及在正常和乙酸诱导的逼尿肌过度活动条件下的尿动力学参数。方法在乌拉坦麻醉的雄性Wistar大鼠中，以5Hz、脉冲宽度200s和大约阈值三倍的幅度刺激胫神经30分钟，以诱导轻微的脚趾运动。通过测量两种不同条件下盆腔节后神经分支的传入神经活动和各种尿动力学参数来研究刺激后的效果：（i）生理盐水填充实验（模拟正常膀胱条件）； (ii) 在醋酸刺激的膀胱中（模拟逼尿肌过度活动）。结果在盐水充盈实验中，胫神经刺激 30 分钟后，膀胱容量、阈压和传入神经活动与刺激前测量值没有显着差异。 0.5%乙酸滴注显着减少膀胱容量并增加传入神经活动。与刺激前的乙酸测量相比，胫神经刺激显着改善了膀胱容量并抑制了传入神经活动。结论胫神经刺激能够通过抑制化学刺激的大鼠膀胱的传入神经活动来显着恢复膀胱容量。本研究提供了重要的基本电生理学证据，以证实胫神经刺激的临床用途用于治疗与逼尿肌过度活动相关的症状。
ObjectivesTo study the post-stimulation effect of tibial nerve stimulation on rat bladder afferent activity, and urodynamic parameters in normal and acetic acid-induced detrusor overactivity conditions.MethodsIn urethane anesthetized male Wistar rats, the tibial nerve was stimulated for 30min at 5Hz, pulse width 200s and amplitude approximately threefold the threshold to induce a slight toe movement. The post-stimulation effect was studied by measuring afferent nerve activity of postganglionic pelvic nerve branches and various urodynamic parameters under two different conditions: (i) in physiological saline filling experiments (simulating normal bladder condition); and (ii) in acetic acid irritated bladders (simulating detrusor overactivity).ResultsAfter 30min of tibial nerve stimulation in saline filling experiments, the bladder capacity, threshold pressure and afferent nerve activity were not significantly different from the prestimulation measurements. The instillation of 0.5% acetic acid significantly reduced the bladder capacity and increased the afferent nerve activity. Tibial nerve stimulation significantly improved the bladder capacity and suppressed the afferent nerve activity compared with prestimulation acetic acid measurements.ConclusionsTibial nerve stimulation is able to significantly restore the bladder capacity by inhibiting afferent nerve activity in chemically irritated rat bladders. The present study provides important basic electrophysiological evidence to substantiate the clinical use of tibial nerve stimulation for treatment of symptoms related to detrusor overactivity.