A computational tool for quantitative analysis of vascular networks.
A computational tool for quantitative analysis of vascular networks.
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DOI：
10.1371/journal.pone.0027385
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Vermeren S
中科院分区：
文献类型：
--
作者：
Zudaire E;Gambardella L;Kurcz C;Vermeren S
血管生成是从预先存在的血管生成成熟的血管网络。血管生成对于生物体的发育、伤口愈合和女性生殖周期至关重要。几种小鼠实验系统非常适合研究发育和病理性血管生成。它们包括胚胎后脑、产后视网膜和尿囊外植体。在这些系统中，通过适当的染色程序然后进行显微镜分析来可视化血管网络。然而，由于缺乏现成的标准化指标和软件分析工具，血管生成的定量评估受到阻碍。非自动化协议正在被广泛使用，它们通常是时间和劳动力密集型的，容易出现人为错误，并且不允许计算复杂的空间度量。我们开发了一款轻量级、用户友好的软件 AngioTool，它可以对显微图像中的血管网络进行快速、无需干预且可重复的量化。 AngioTool 计算多个形态和空间参数，包括血管网络覆盖的面积、血管数量、血管长度、血管密度和腔隙性。此外，AngioTool 还可以计算所谓的“分支指数”（分支点/单位面积），从而测量感兴趣样本的发芽活动。我们使用胚胎鼠后脑、产后视网膜和尿囊外植体的图像验证了 AngioTool。 AngioTool 是开源的，可以免费下载。
Angiogenesis is the generation of mature vascular networks from pre-existing vessels. Angiogenesis is crucial during the organism' development, for wound healing and for the female reproductive cycle. Several murine experimental systems are well suited for studying developmental and pathological angiogenesis. They include the embryonic hindbrain, the post-natal retina and allantois explants. In these systems vascular networks are visualised by appropriate staining procedures followed by microscopical analysis. Nevertheless, quantitative assessment of angiogenesis is hampered by the lack of readily available, standardized metrics and software analysis tools. Non-automated protocols are being used widely and they are, in general, time - and labour intensive, prone to human error and do not permit computation of complex spatial metrics. We have developed a light-weight, user friendly software, AngioTool, which allows for quick, hands-off and reproducible quantification of vascular networks in microscopic images. AngioTool computes several morphological and spatial parameters including the area covered by a vascular network, the number of vessels, vessel length, vascular density and lacunarity. In addition, AngioTool calculates the so-called “branching index” (branch points / unit area), providing a measurement of the sprouting activity of a specimen of interest. We have validated AngioTool using images of embryonic murine hindbrains, post-natal retinas and allantois explants. AngioTool is open source and can be downloaded free of charge.
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影响因子：
10.5
作者：
Ruhrberg, C;Gerhardt, H;Shima, DT
通讯作者：
Shima, DT
影响因子：
64.5
作者：
Knight, Zachary A.;Gonzalez, Beatriz;Shokat, Kevan M.
通讯作者：
Shokat, Kevan M.
DOI：
10.1016/j.bbrc.2009.07.108
发表时间：
2009-10-02
影响因子：
3.1
作者：
Kanemura, Hoshimi;Satoh, Takaya;Kataoka, Tohru
通讯作者：
Kataoka, Tohru
影响因子：
64.8
作者：
Uehata, M;Ishizaki, T;Narumiya, S
通讯作者：
Narumiya, S
影响因子：
11.2
作者：
Croft, DR;Sahai, E;Olson, MF
通讯作者：
Olson, MF