IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
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发表时间：
1999-05
影响因子：
4.4
通讯作者：
M. Yamaguchi;K. Sayama;K. Yano;C. Lantz;N. Noben-Trauth;C. Ra;J. J. Costa-J.;S. Galli
中科院分区：
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作者：
M. Yamaguchi;K. Sayama;K. Yano;C. Lantz;N. Noben-Trauth;C. Ra;J. J. Costa-J.;S. Galli
我们研究了IgE和IL-4对体外培养的脐血单个核细胞分化的人肥大细胞表面Fc epsilon RI表达的影响。我们发现，免疫球蛋白E(5微克/毫升)比IL-4(0.1-100 ng/毫升)更显著地促进Fc epsilon RI的表面表达；在分化的小鼠肥大细胞中也得到了类似的结果。然而，IL-4与IgE协同作用，在这些脐带血来源的人肥大细胞以及IL-4-/-或IL-4+/+小鼠来源的小鼠腹膜肥大细胞中增强Fc epsilon RI的表达。我们还发现：1)IgE依赖的Fc epsilon RI表达的增强与这些人肥大细胞分泌组胺、PGD2和白三烯C4的能力显著增强有关，在随后用IgE被动增敏和抗IgE攻击时；2)IL-4预孵育促进这些细胞的IgE依赖介质的分泌，即使对Fc epsilon RI表面表达没有显著影响；3)当IL-4与IgE一起使用时，人肥大细胞的IgE依赖介质的分泌水平高于单独用IgE预孵育的细胞；4)不同来源的脐血来源的体外培养的人肥大细胞，无论是在基线条件下还是在与IgE和/或IL-4预先孵育后，在抗IgE刺激下，组胺和脂质介质释放的大小和模式都不同。
We investigated the effects of IgE versus IL-4 on Fc epsilon RI surface expression in differentiated human mast cells derived in vitro from umbilical cord blood mononuclear cells. We found that IgE (at 5 micrograms/ml) much more strikingly enhanced surface expression of Fc epsilon RI than did IL-4 (at 0.1-100 ng/ml); similar results were also obtained with differentiated mouse mast cells. However, IL-4 acted synergistically with IgE to enhance Fc epsilon RI expression in these umbilical cord blood-derived human mast cells, as well as in mouse peritoneal mast cells derived from IL-4-/- or IL-4+/+ mice. We also found that: 1) IgE-dependent enhancement of Fc epsilon RI expression was associated with a significantly enhanced ability of these human mast cells to secrete histamine, PGD2, and leukotriene C4 upon subsequent passive sensitization with IgE and challenge with anti-IgE; 2) preincubation with IL-4 enhanced IgE-dependent mediator secretion in these cells even in the absence of significant effects on Fc epsilon RI surface expression; 3) when used together with IgE, IL-4 enhanced IgE-dependent mediator secretion in human mast cells to levels greater than those observed in cells that had been preincubated with IgE alone; and 4) batches of human mast cells generated in vitro from umbilical cord blood cells derived from different donors exhibited differences in the magnitude and pattern of histamine and lipid mediator release in response to anti-IgE challenge, both under baseline conditions and after preincubation with IgE and/or IL-4.