A catalog for transcripts in the venom gland of the Agkistrodon acutus:: Identification of the toxins potentially involved in coagulopathy
A catalog for transcripts in the venom gland of the Agkistrodon acutus:: Identification of the toxins potentially involved in coagulopathy
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DOI：
10.1016/j.bbrc.2006.01.006
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发表时间：
2006-03-10
影响因子：
3.1
通讯作者：
Yan, GM
中科院分区：
文献类型：
--
作者：
Liu, QH;Zhang, XW;Yan, GM
尖吻蝮蛇（Agkistrodon acutus）是一种特有的蝮蛇属（Agkistrodon halys），仅分布于我国南方。除了在越南的几个例外。它是传统中药中使用的珍贵元素。为了获得五步蛇毒腺基因表达的全貌，构建了五步蛇毒腺非标准化cDNA文库。对8696个高质量的5'端表达序列标签（EST）进行测序分析。将初始序列组装成2855个簇。在这些聚类中，只有45.60%的聚类与已知序列相匹配，54.40%的聚类与GenBank中的任何已知序列都不匹配。除了推测的细胞蛋白（1184簇），其余118簇（40.16%的EST）对应的序列与不同的毒素功能。从表达丰度来看，主要毒素成分为金属蛋白酶（32.08%）和C型凝集素（5.22%），以及其他成分，包括缓激肽增强肽（0.90%），丝氨酸蛋白酶（0.51%）、核苷酸酶和核酸酶（0.41%）、磷脂酶A2（0.30%）、去整合素（0.05%）、类精氨酸分子（0.06%）和其它蛋白质（0.63%）。这些成分中的大多数被认为是A.急性咬伤因此，我们已经产生了一个全面的目录A。迄今为止所描述的尖吻毒腺。从非常专门的分泌组织，特别是那些参与凝血病的基因表达，可以调查和发现新的毒素提供重要的信息。(c)2006年爱思唯尔公司All rights reserved.
Agkistrodon acutus is a special agkistrodon halys, only distributed in Southern China.. with a few exceptions in Vietnam. It is a cherished element used in traditional Chinese medicine. In order to produce a global panorama of gene expression in the Agkistrodon acutus venom gland, a non-normalized cDNA library was Constructed,. and 8696 high quality 5' end expressed sequenced tags (ESTs) were sequenced and analyzed. The initial sequences were assembled into 2855 clusters. Of these clusters, only 45.60% clusters matched known sequence and 54.40% had no match to any known sequence in GenBank. Except for putative cellular proteins (1184 clusters), the remaining 118 clusters (40.16% of all ESTs) corresponded to sequences associated with diverse toxin function. According to expression abundance, the major toxin components were metalloproteinases (32.08%) and C-type lectin (5.22%), and other components including bradykinin-potentiating peptide (0.90%), serine proteases (0.51%), nucleotidase and nuclease (0.41%), phospholipase A2 (0.30%), disintegrin (0.05%), cytokine-like molecules (0.06%), and other proteins (0.63%). The majority of these components are thought to be responsible for coagulopathy after A. acutus bites. We have therefore generated a comprehensive catalog of the A. acutus venom gland described so far. Gene expression from the very specialized secretory tissue, especially for those involved in coagulopathy, can be surveyed and provide important information in finding novel toxins. (c) 2006 Elsevier Inc. All rights reserved.