Endometrial stromal cells regulate epithelial cell growth in vitro:: a new co-culture model
Endometrial stromal cells regulate epithelial cell growth in vitro:: a new co-culture model
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DOI：
10.1093/humrep/16.5.836
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发表时间：
2001-05-01
影响因子：
6.1
通讯作者：
Lessey, BA
中科院分区：
文献类型：
--
作者：
Arnold, JT;Kaufman, DG;Lessey, BA
间质或间质中的旁分泌效应对上皮细胞功能和形态发生的调节作用已经在体内研究中得到了很好的证实。对这些关系的更完整的理解被推迟了，部分原因是缺乏适当的共同文化模式。在这项研究中，我们描述了一种共培养模型，该模型证明了在体外可以重建正常的旁分泌关系，并且人子宫内膜基质细胞调节原代人子宫内膜上皮细胞的生长和分化。在对基底膜提取物Matrigel(R)的反应中，发现基质细胞和上皮细胞的增殖存在有趣的差异。基质细胞在体外暴露于Matrigel(R)可增强这些细胞的旁分泌能力。当上皮细胞与镶嵌在Matrigel(R)中的基质细胞接触时，与对照组相比，上皮细胞的生长受到65-80%的抑制。与Matrigel(R)接触的基质细胞也调节上皮细胞分化，如诱导糖蛋白表达所示。这些共培养研究为研究子宫内膜间质和上皮细胞及其环境之间的细胞相互作用以及了解调控子宫内膜等复杂组织内细胞正常生长和分化的分子基础提供了一种很有前途的方法。
The regulation of epithelial cell function and morphogenesis by the paracrine effecters from the mesenchyme or stroma has been well established using in-vivo studies. A more complete understanding of these relationships has been delayed due, in part, to a lack of appropriate co-culture models. in this study, we describe a co-culture model which demonstrates that normal paracrine relationships can be reconstituted in vitro and that human endometrial stromal cells regulate both growth and differentiation of primary human endometrial epithelial cells. Interesting differences in the proliferation of stromal and epithelial cells were noted in response to the basement membrane extract, Matrigel (R). Exposure of stromal cells to Matrigel (R) enhanced the paracrine capacity of these cells in vitro. When epithelial cells were co-cultured in contact with stromal cells embedded in Matrigel (R), epithelial cell growth was inhibited by 65-80% compared to controls. Stromal cells in contact with Matrigel (R) also regulated epithelial cell differentiation, as shown by induction of glycodelin expression. These co-culture studies show great promise as a method to investigate the cellular interactions between endometrial stromal and epithelial cells and their environment and to understand the molecular basis for the regulation of normal growth and differentiation of cells within complex tissues such as the endometrium.