Improvement of biodiesel production by lipozyme TL IM-catalyzed methanolysis using response surface methodology and acyl migration enhancer.
Improvement of biodiesel production by lipozyme TL IM-catalyzed methanolysis using response surface methodology and acyl migration enhancer.
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DOI：
10.1016/j.biortech.2007.12.062
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发表时间：
2008-10
影响因子：
11.4
通讯作者：
Yan Wang;Hong Wu;Min-hua Zong
中科院分区：
文献类型：
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作者：
Yan Wang;Hong Wu;Min-hua Zong
采用响应面分析法（RSM）和中心组合旋转设计（CCRD）研究了廉价的嗜热脂肪酶TL IM催化玉米油制备生物柴油的工艺条件，叔丁醇与油的比例（v/v）和甲醇与油的比例（mol/mol）对甲醇分解的甲酯（ME）产率的影响。最佳反应条件为：酶量25.9U/g，叔丁醇与油的体积比0.58，甲醇与油的摩尔当量分别为0.5、0.5、2.8，反应时间分别为0、2、4 h，此时ME产率为85.6%，与预测值85.0%非常接近。反应12 h后即可得到。在最佳工艺条件下，以废油为原料，ME产率可达93.7%。添加酰基迁移促进剂三乙胺（TEA）可有效提高玉米油醇解的ME产率，ME产率可达92.0%。
The process of biodiesel production from corn oil catalyzed by lipozyme TL IM, an inexpensive 1,3-position specific lipase from Thermomyces lanuginosus was optimized by response surface methodology (RSM) and a central composite rotatable design (CCRD) was used to study the effects of enzyme dosage, ratio of t-butanol to oil (v/v) and ratio of methanol to oil (mol/mol) on the methyl esters (ME) yield of the methanolysis. The optimum combinations for the reaction were 25.9U/goilof enzyme, 0.58 volume ratio of t-butanol to oil and 0.5, 0.5, 2.8 molar equivalent of methanol to oil added at the reaction time of 0, 2, and 4h, respectively, by which a ME yield of 85.6%, which was very close to the predicted value of 85.0%, could be obtained after reaction for 12h. Waste oil was found to be more suitable feedstock, and could give 93.7% ME yield under the optimum conditions described above. Adding triethylamine (TEA), an acyl migration enhancer, could efficiently improve the ME yield of the methanolysis of corn oil, giving a ME yield of 92.0%.