Monitoring expression profiles of Arabidopsis genes during cold acclimation and deacclimation using DNA microarrays
Monitoring expression profiles of Arabidopsis genes during cold acclimation and deacclimation using DNA microarrays
复制标题
DOI：
10.1007/s10142-005-0014-z
复制
发表时间：
2006-07-01
影响因子：
2.9
通讯作者：
Shinozaki, Kazuo
中科院分区：
文献类型：
--
作者：
Oono, Youko;Seki, Motoaki;Shinozaki, Kazuo
为了阐明高等植物冷应激反应的分子机制，有必要对冷驯化和脱驯化过程中的基因表达谱进行比较分析。我们使用两种微阵列系统（7K RAFL cDNA 微阵列和 Agilent 22K 寡核苷酸阵列）分析了冷驯化和去驯化（从冷应激中恢复）过程中的基因表达谱。通过两种微阵列分析，我们鉴定出在去驯化期间有 292 个基因上调和 320 个基因下调，以及在冷驯化期间有 445 个冷上调基因和 341 个冷下调基因。发现许多在去驯化过程中上调的基因在冷驯化过程中下调，反之亦然。去驯化过程中上调的基因分为（1）参与进一步调节信号转导和基因表达的调节蛋白和（2）参与冷应激诱导损伤和植物生长恢复过程的功能蛋白。我们还应用表达谱研究来鉴定参与碳水化合物和氨基酸生物合成的关键基因，这些基因已知在冷适应中发挥重要作用。我们比较了脱驯化过程中受调节的基因与脱水后补液过程中受调节的基因，以讨论每个恢复过程的相似性和差异。
A comparative analysis of gene expression profiles during cold acclimation and deacclimation is necessary to elucidate the molecular mechanisms of cold stress responses in higher plants. We analyzed gene expression profiles in the process of cold acclimation and deacclimation (recovery from cold stress) using two microarray systems, the 7K RAFL cDNA microarray and the Agilent 22K oligonucleotide array. By both microarray analyses, we identified 292 genes up-regulated and 320 genes down-regulated during deacclimation, and 445 cold up-regulated genes and 341 cold down-regulated genes during cold acclimation. Many genes up-regulated during deacclimation were found to be down-regulated during cold acclimation, and vice versa. The genes up-regulated during deacclimation were classified into (1) regulatory proteins involved in further regulation of signal transduction and gene expression and (2) functional proteins involved in the recovery process from cold-stress-induced damages and plant growth. We also applied expression profiling studies to identify the key genes involved in the biosynthesis of carbohydrates and amino acids that are known to play important roles in cold acclimation. We compared genes that are regulated during deacclimation with those regulated during rehydration after dehydration to discuss the similarity and difference of each recovery process.