Methylnigakinone content determination and geographical origin discrimination for P. quassioides via fluorescence fingerprint and principal component analyses
Methylnigakinone content determination and geographical origin discrimination for P. quassioides via fluorescence fingerprint and principal component analyses
复制标题
荧光指纹图谱和主成分分析测定苦木酮含量及产地判别
DOI：
10.1016/j.jpba.2022.114932
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发表时间：
2022
影响因子：
3.4
通讯作者：
Karasawa Koji
中科院分区：
文献类型：
--
作者：
Hamazaki Yasunori;Kato Masaru;Karasawa Koji
苦木被用作一种甜味的胃药。因为它是从不同地理区域获得的天然产品，所以生产区域很重要。被用作一种生药的拟苦木。本研究建立了一种快速、简便、快速的测定三七中主要活性成分之一甲基黑木酮含量的方法。quassioides，和一个协议，用于区分这种天然产品的地理来源，使用荧光指纹分析和主成分分析（PCA）。由于甲基黑木酮具有荧光性（激发波长：352 nm，发射波长：458 nm），因此可在0.1 - 1 μ g/mL浓度范围内测定该分子的含量。使用所开发的方法获得的甲基黑木酮的定量结果与从HPLC分析获得的结果相似。此外，还对P. quassioides产生了一个分数图与三个不同的地理起源（九州岛（日本），四国岛（日本），和中国）绘制的地区。因此，有可能区分的地理起源的P。拟似样本开发的方法简单，快速，对环境的影响最小。因此，所建立的方法将有助于确定P的来源。苦木
Picrasmaquassioidesis used as a bittersweet stomach medicine. Because it is a natural product obtained from various geographical regions, the production area is important whenP. quassioidesis used as a crude drug. Herein, we developed a method to determine the content of methylnigakinone, one of the major active ingredients inP. quassioides, and a protocol for discriminating the geographical origin of this natural product using a fluorescence fingerprint analysis and principal component analysis (PCA). Because methylnigakinone is fluorescent (excitation wavelength: 352 nm, emission wavelength: 458 nm), the content of this molecule can be determined in the concentration range of 0.1–1 μg/mL. The quantification results of methylnigakinone obtained using the developed method were similar to those obtained from an HPLC analysis. Furthermore, the PCA of the fluorescence fingerprint ofP. quassioidesproduced a score plot with the three different geographical origins (Kyushu island (Japan), Shikoku island (Japan), and China) plotted in the regions. Thus, it was possible to discriminate the geographical origin of theP. quassioidessamples. The developed method is simple, quick, and has a minimal environmental impact. Therefore, the developed method will be useful for confirming the origin ofP. quassioides.