Electronically facilitated transdermal delivery of human parathyroid hormone (1-34).
Electronically facilitated transdermal delivery of human parathyroid hormone (1-34).
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电子促进人甲状旁腺激素的透皮递送 (1-34)。
DOI：
10.1016/s0378-5173(03)00337-5
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发表时间：
2003
影响因子：
5.8
通讯作者：
Singh,Jagdish
中科院分区：
文献类型：
--
作者：
Medi,BabuM;Singh,Jagdish
本文研究了人甲状旁腺激素（1 - 34）的电促透皮给药。研究了离子导入电流密度、脉冲电压以及电导入后离子导入对人甲状旁腺素（1-34）体外透皮吸收的影响。0.5mA/cm ~ 2电流密度的离子导入使hPTH（1-34）的流量显著增加（P<0.05）。100、200和300 V的电穿孔脉冲与0.5mA/cm 2的被动以及离子电渗通量相比显著增加（P<0.05）hPTH（1-34）的通量。hPTH（1-34）的电穿孔通量与脉冲幅度呈线性关系（R2=0.97）。皮肤的主要屏障，角质层，被发现扰乱后的脉冲，明显的光学显微镜研究。应用电穿孔脉冲，然后离子电渗进一步增加了几倍的通量。与单独使用相应脉冲的通量相比，使用100 V和300 V的电穿孔脉冲以及随后以0.2mA/cm 2的离子导入法的hPTH（1-34）通量分别高出10倍和5倍。这显示了离子电渗与电穿孔组合对hPTH（1-34）的皮肤渗透性的协同作用。结果表明，使用电穿孔后的离子导入法设计hPTH（1-34）的受控透皮给药系统的可能性。
Electronically facilitated transdermal delivery of human parathyroid hormone (1–34), hPTH (1–34), was investigated in vitro, using dermatomed porcine skin. The effect of iontophoretic current density, electroporative pulse voltages and also electroporation followed by iontophoresis was investigated on the in vitro percutaneous absorption of hPTH (1–34). Iontophoresis at 0.5mA/cm2current density significantly enhanced (P<0.05) the flux of hPTH (1–34) in comparison to passive flux. Electroporation pulses of 100, 200 and 300V significantly increased (P<0.05) the flux of hPTH (1–34) in comparison with the passive as well as iontophoretic flux at 0.5mA/cm2. The electroporative flux of hPTH (1–34) was found to vary linearly (R2=0.97) with the pulse amplitude. The principal barrier of the skin, stratum corneum, was found perturbed following the pulses as evident by light microscopy studies. The application of electroporation pulses followed by iontophoresis further increased the flux by several fold. The flux of hPTH (1–34) with the electroporation pulses of 100 and 300V followed by iontophoresis at 0.2mA/cm2was 10- and 5-fold higher, respectively, in comparison to the flux with corresponding pulses alone. This shows the synergistic effect of iontophoresis in combination with electroporation on skin permeability of hPTH (1–34). The results indicate the possibility of designing controlled transdermal delivery systems for hPTH (1–34) using electroporation followed by iontophoresis.