Isolation of a gibberellin 20-oxidase cDNA from and characterization of its expression in chrysanthemum
Isolation of a gibberellin 20-oxidase cDNA from and characterization of its expression in chrysanthemum
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从菊花中分离赤霉素 20-氧化酶 cDNA 及其在菊花中的表达特征
DOI：
10.1111/j.1439-0523.2009.01736.x
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发表时间：
2010-12-01
期刊：
影响因子：
2
通讯作者：
Guan, Z.
中科院分区：
文献类型：
--
作者：
Miao, H.;Jiang, B.;Guan, Z.
采用PCR方法从菊花中克隆了GA 20-氧化酶的全长cDNA。该基因编码一个377个氨基酸残基的多肽，与莴苣GA 20 ox同源性为87%。Southern杂交分析表明，六倍体菊花基因组可能含有几个GA 20 ox拷贝。DgGA 20 ox基因在叶、茎、花和根中表达，但在幼叶中表达最强。在第4节间施用GA生物合成抑制剂多效唑可增加DgGA 20 ox的表达，而外源GA（3）则可降低DgGA 20 ox的表达，这支持了GA 20 ox基因的表达水平受生理活性GA的局部水平调节这一普遍观察到的现象。讨论了DgGA 20 ox基因与菊花育种的相关性。
P>A PCR strategy was used to isolate a full-length GA20-oxidase cDNA from chrysanthemum. The gene putatively encodes a 377 residue polypeptide, which shares 87% homology with lettuce GA20ox. A Southern blot analysis indicated that the hexaploid chrysanthemum genome probably contains several GA20ox copies. The DgGA20ox gene is expressed in the leaf, stem, flower and root, but most strongly in the young leaf. The application of the GA biosynthesis inhibitor paclobutrazol to the fourth internode increased the expression of DgGA20ox, while exogenously applied GA(3) decreased it. This supports the commonly observed phenomenon that the expression level of GA20ox genes is regulated by the local level of physiologically active GA. The relevance of the DgGA20ox in relation to chrysanthemum breeding was discussed as well.