The light-harvesting complex of photosystem I in Chlamydomonas reinhardtii: protein composition, gene structures and phylogenic implications.
The light-harvesting complex of photosystem I in Chlamydomonas reinhardtii: protein composition, gene structures and phylogenic implications.
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DOI：
10.1093/pcp/pch013
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发表时间：
2004-02
影响因子：
4.9
通讯作者：
Ryutaro Tokutsu;H. Teramoto;Yuichiro Takahashi;T. Ono;J. Minagawa
中科院分区：
文献类型：
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作者：
Ryutaro Tokutsu;H. Teramoto;Yuichiro Takahashi;T. Ono;J. Minagawa
与光系统I（PSI）相关的捕光叶绿素a/b结合蛋白（LHCI）和编码这些蛋白的基因已经在单细胞绿色衣藻（Chlamyeliumreinhardtii）中被表征，扩展了PSII-LHCII的先前研究[Teramoto等（2001）Plant Cell Physiol.42：849]。为了在类囊体膜中分配LHCI蛋白，纯化了保留所有主要LHCI蛋白的PSI-LHCI超复合物。7个不同的LHCI蛋白从纯化的超复合物解析的高分辨率SDS聚丙烯酰胺凝胶电泳，并确定其N-末端氨基酸序列。一个LHCI蛋白（带e）是新发现的，虽然其他六个LHCI蛋白对应于以前报道的。新分离编码这7种LHCI蛋白的基因组克隆，并测定核苷酸序列。对Lhc基因家族所有成员的综合表征表明，LHCI蛋白比LHCII更高度分化，表明LHCI中蛋白组分的功能分化。构建了C. reinhardtii和拟南芥（Arabidopsis thaliana）或Galdieria sulphuraria的那些，以评估进化关系。系统发育分析表明：（1）.绿色藻类LHCI和LHCII蛋白质彼此之间的关系比与红藻中的LHCI蛋白质的关系更密切（2）。绿色藻类和高等植物拥有七种常见的LHC蛋白谱系，以及（3）。I型和III型LHCI蛋白在绿色藻类和高等植物之间是保守的，而II型和IV型不是。这些研究结果进行了讨论的背景下，多个不同的天线复合体的演变。
Light-harvesting chlorophyll a/b-binding proteins (LHCI) associated with photosystem I (PSI) and the genes encoding these proteins have been characterized in the unicellular green alga Chlamydomonas reinhardtii, extending previous studies of the PSII-LHCII [Teramoto et al. (2001) Plant Cell Physiol. 42: 849]. In order to assign LHCI proteins in the thylakoid membranes, the PSI-LHCI supercomplex that retains all of the major LHCI proteins was purified. Seven distinct LHCI proteins were resolved from the purified supercomplex by a high-resolution SDS polyacrylamide gel electrophoresis, and their N-terminal amino acid sequences were determined. One LHCI protein (band e) was newly found, although the other six LHCI proteins corresponded to those previously reported. Genomic clones encoding these seven LHCI proteins were newly isolated and the nucleotide sequences were determined. A comprehensive characterization of all members of Lhc gene family in this alga revealed that LHCI proteins are more highly diverged than LHCII, suggesting functional differentiation of the protein components in LHCI. Neighbor joining trees were constructed for LHC proteins from C. reinhardtii and those of Arabidopsis thaliana or Galdieria sulphuraria to assess evolutionary relationships. Phylogenetic analysis revealed that (1). green algal LHCI and LHCII proteins are more closely related to one another than to LHCI proteins in red algae, (2). green algae and higher plants possess seven common lineages of LHC proteins, and (3). Type I and III LHCI proteins are conserved between green algae and higher plants, while Type II and IV are not. These findings are discussed in the context of evolution of multiple diverse antenna complexes.