Elevated CO2 delayed the chlorophyll degradation and anthocyanin accumulation in postharvest strawberry fruit
Elevated CO2 delayed the chlorophyll degradation and anthocyanin accumulation in postharvest strawberry fruit
复制标题
二氧化碳浓度升高延缓采后草莓果实叶绿素降解和花青素积累
DOI：
10.1016/j.foodchem.2019.01.150
复制
发表时间：
2019-07-01
期刊：
影响因子：
8.8
通讯作者：
Luo, Zisheng
中科院分区：
文献类型：
--
作者：
Li, Dong;Zhang, Xiaochen;Luo, Zisheng
颜色是决定消费者是否接受水果的重要品质属性。在草莓果实中施用高浓度CO2，探讨其对叶绿素分解代谢和花青素合成的影响。结果表明，20% CO2处理延迟了草莓果实a*和b*值的变化。通过抑制叶绿素酶活性，下调fachlb还原酶、FaPAO和FaRCCR的表达，减缓了CO2处理果实叶绿素的降解。此外，在20% CO2的作用下，花青素浓度降低，PAL、C4H、4CL和CHS活性降低。同时，qRT-PCR分析显示，在CO2胁迫下，苯丙素途径和类黄酮生物合成途径相关的13个基因也出现了下调。然而，当去除高浓度的CO2时，没有观察到对色素代谢的残留影响。我们的研究为采后CO2升高在色素代谢中的调节作用提供了新的见解。
Colour is an important quality attribute for the consumer's acceptability of fruit. Elevated CO2 was applied to strawberry fruit to explore its influence on chlorophyll catabolism and anthocyanin synthesis. The results showed that 20% CO2 delayed the changes of a* and b* values in strawberry fruit. The degradation of chlorophyll was delayed in CO2 treated fruit by inhibiting the activities of chlorophyllase and down-regulating the expression of FaChl b reductase, FaPAO and FaRCCR. In addition, lower concentration of anthocyanins and lower activity of PAL, C4H, 4CL and CHS were recorded under the effect of 20% CO2. Meanwhile, qRT-PCR analysis showed that 13 genes involved in the phenylpropanoid pathway and the flavonoid biosynthesis pathway were also down-regulated under CO2 stress. However, no residual effect on pigment metabolism was observed when elevated CO2 was removed. Our study provided new insights into the regulation of elevated CO2 in the role of pigment metabolism in postharvest.