The selective eosinophil chemotactic activity of histamine.
The selective eosinophil chemotactic activity of histamine.
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DOI：
10.1084/jem.142.6.1462
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发表时间：
1975-12-01
期刊：
影响因子：
--
通讯作者：
Kaplan AP
中科院分区：
文献类型：
--
作者：
Clark RA;Gallin JI;Kaplan AP
当在改良的Boyden室趋化测定系统中使用超过20%的嗜酸性粒细胞时，二磷酸组胺显示出从混合粒细胞群体中选择性地吸引人嗜酸性粒细胞。组胺的这种作用通过与二胺氧化酶（组胺酶）孵育而消除，并通过L-组氨酸的脱羧作用产生。在3 × 10（-7）M和1.25 × 10（-6）M之间观察到嗜酸性粒细胞迁移的线性剂量依赖性增加，而较高浓度的组胺抑制嗜酸性粒细胞的迁移。组胺的引诱活性不受H-1或H-2受体拮抗剂的抑制，然而，在较高的组胺浓度下观察到的迁移抑制被甲硫胺，H-2受体拮抗剂逆转。组胺对嗜酸性粒细胞迁移的影响可使用三种不同的试验证明：（a）计数已经穿过5 μ m孔、12 μ m厚的聚碳酸酯过滤器的细胞，（B）计数已经迁移不同距离进入3 μ m孔、145 μ m硝酸纤维素过滤器的细胞，或（c）使用15 Cr标记的细胞测量已经穿过上部聚碳酸酯过滤器并迁移到下部硝酸纤维素过滤器中的细胞的数量。组胺增强嗜酸性粒细胞迁移的能力被证明是依赖于浓度梯度的存在;组胺没有引起随机运动的剂量依赖性增加。此外，嗜酸性粒细胞与组胺的预孵育使细胞对组胺或C5 a的进一步刺激失活。它的结论是，在低剂量组胺是人类嗜酸性粒细胞的化学引诱剂，而在较高剂量组胺抑制嗜酸性粒细胞迁移。这些观察结果可能与速发型超敏反应中嗜酸性粒细胞的流入和定位有关。
Histamine diphosphate was shown to selectively attract human eosinophils from mixed granulocyte populations when over 20% eosinophils were used in a modified Boyden chamber chemotactic assay system. This effect of histamine is abolished by incubation with diamine oxidase (histaminase) and was generated by decarboxylation of L- histidine. A linear dose dependent increase in eosinophil migration was observed between 3 X 10(-7) M and 1.25 X 10(-6) M, while higher concentrations of histamine inhibited the migration of eosinophils. The attractant activity of histamine was not inhibited by H-1 or H-2 receptor antagonists, however, the inhibition of migration observed at higher histamine concentrations was reversed by metiamine, an H-2 receptor antagonist. The effects of histamine upon eosinophil migration were demonstrable using three different assays: (a) counting cells that had traversed 5-mum pore, 12-mum thick polycarbonate filters, (b) counting cells that had migrated various distances into a 3-mum pore, 145-mum cellulose nitrate filters, or (c) measuring the number of cells that had traversed an upper polycarbonate filter and migrated into a lower cellulose nitrate filter using 15Cr-labeled cells. The ability of histamine to enhance eosinophil migration was shown to be dependent upon the presence of a concentration gradient; histamine did not cause a dose-dependent increase in random motility. Furthermore, preincubation of the eosinophils with histamine deactivate the cells to further stimulation by histamine or by C5a. It is concluded that in low doses histamine is a chemoattractant for human eosinophils, while in higher doses histamine inhibits eosinophil migration. These observations may relate to the influx and localization of eosinophils in immediate hypersensitivity reactions.